XB-CLONE-4350726
Clone Name: IMAGE:7025966
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Gene:
hoxc12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785343 [+]
taattaatcccccccccccccagaagctcctggtcaggcccgcggaaatgggagaacataatcttcttaatcccgggtttgtgggacctctggtgaatat
ccataccggggacaccttctatttccccaatttcaggacccccggcgggcagttacctggcttgccctctctctcttatccaagaagggaaaatgtctgt
tctctgccatgggcatccacagagccgtgcaacggatacccccagccctatctcagcagcccggtctccattaacccttccttcaccacggcttgtgatc
tggcccgagtggaagagacccaatgccattaccgggaggggtgcaaccccaacaacccctgcctaaaaagggagaatgcgccgctgcccctggaaagcgg
gatccatatcgccatgcacaggattttgcagaaatcctaattaccccgctgtggaattaagtatcataagaattatctctcccagttcatccctccgaag
agaaaaccccagcatagccctatgacttatttaggcggaccttattaaaaccccgaaacccacaaatatattcacaaaaggggatttattcccatttcac
atgcagaggcaagcgtatattttttatataaaagctggccaagaaaaaaaaagactgaggacaaaccctattttcagtcagccttaaaaacgatcggcac
ctctaattcttacgcttaccccctttttatgcatcggagaatataacactctgccacatttattcgaaccagaaatttcctattctacccccgcaccgtt
gtatttacagttatagagcgcccacgcccccgatttagaagaaacagacacactacctggtattatatacaactgcccggcgccacctcttg
3' EST: CF785344 [+]atccaagttttaacggaattcgtaacattttttttgtttttatgcctgtcaatttaatgaactgattctttctaaatatagatcaaaataaaagtattaa
tggctaacaaaacctcattcattactaatatgggggctgtagaaagttcgtattcgctttaatggactaacgcatgtataagataaaataaatatatatt
tgtgtttgtacatatatagacagaaaataaaaaagaaagcctttgaaataggcgtaggcaggcaattaccccacttgctgcagatgactgtctgctccag
attggcgattatatatatatatatatatatatatatatatacatataatgctttctataaaatgcaacattatttttatactttggttaaataccctcct
ttacaattagaaattacaaagagaaaggaaaggaaaccttctcagttacatgcttggtatcctgcactccgagcttttacagattctacccatatttgca
gcactaagggcaagtaggcaagttaatgaccattcttgatgctataataataaagaaatttgtcagccaaagactcactgaaaacataatactattacta
tcaagcttccttactgaacaacgtttaactgcgtgtcaagttttgtctaggtttctgccaatatcttgccaacggagacagacaaatgagacaagatatt
gcatccttgagtattggtgagaataaaagccctattgtggaaagaaaaaatattngcatgtga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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