XB-CLONE-4351572
Clone Name: IMAGE:7027063
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Gene:
pa2g4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784807 [+]
taattaatccccccccccccccccgctctctgcccgccgcctccgctcgctgctacagccgctgccactgctgctactgaggacgggctgaaagagctgt
cggaggccacaaacatgtcgggggacgaagaacagcaggagcagaccatcgcggaggacctggtggtcactaagtacaagatggggggcgacattgccaa
cagggtactgcgcactctggtggatgcagcaacagctggggcttcactccttaatttgtgtgagaagggagatgcaatgattatggaggaaacgggcaaa
atcttcaaaaaggagaaggaaatgaaaaaagggattgcttttccaacgagtatatctgtaaataattgcgtgtgtcatttctcacctctgaaaagtgacc
aagattatccgcttaaggatggcgatctggtgaagattgatctaggagttcatgtggatggctttattgctaatgttgcccacagctttgttgttggagc
ctcaaaggagtgtccggtgactggacgtaaagccgatgtcatcaaagcagcccatctgtgtggagaagccgctttacgtcttgtgaaaccaggaaatcag
aactcgcaagtgactgaagcatggaacaaaatttctccatcttttaaatgtactcccatagaaggaatgctttcccacccgctaaagcagcttgtcattt
gatgggcaaaaagaccatcctttttcaaccccctctggaccagcaaaattaaaggacccctgcataaaagccccacatttttaagggttcccccggaaaa
agcttaatggccccggaaaaaaagcgttcccttaaattaat
3' EST: CF784806 [+]tttttttttttttttgggttttccgacttgctgaactttgcagaagtgccttcaactcggaatcttgcacctcaagctcagatttgtacatatcaggttc
aaaggggccgctagttatcctcataggaccattgggcattaagagaacagtgaacttgaactgagctacaaattcgccctctttttcatagagaacattg
aaaggttggagaagctcatgcttggcgcattccacaactcccattcttgccttcttttcatcctcaaatgccctgagagtaaatggcattgcagcgaagc
gcctctcgacttcgctgaaaaaggcacgggaggttttcattttcaggccatactgcttagtggggtcccgtttgtaaattgttgtgcgctgcccagcgtc
ctttgcctttccctctccagtgctaataagaacatctacagcataaacttcgtggacctcaaattctgctttttcatggtctttcttttgctggtcagtg
gggttctgaatgatggtcttctcgccatcaatgacatgctgctttagctggtgagaaagcattccttctattggagtacatttaaaagatggagaaattt
tattccatgcttcagtcacttgcgagttctgatttcctggtttcacaagacgtaaagcggcttctccacacagatgggctgctttgatgacatcggcttt
acgtccagtcaccggacactccctttgaggctccaacaacaaagctgtgggcaacattagcaataaagccatccacatgaaactcctagatcattctttc
acc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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