XB-CLONE-4352427
Clone Name: IMAGE:7027393
|
Gene:
smn1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784256 [+]
taattaatcccccccccccccgaatacggaagccttaagtgttcaattagtgtatgcgtttagtattgcgagaagtagcgtgtctatggcaggctcggag
gacagtggggaagtgctgttcagacgtggagccggccagagtgatgactctgatatctgggacgatacagctctcattaaagcatatgataaagcagtgt
cttcttttaagaaggcactgaaaaatggggattgtacagttagtgcagaagcagaagaaaaaattcccaggaccaaaaggaaaaataataagaagaatag
aagcaggaagaagtgtaatgccgctccactgaaaaagtggagagtaggcgacacgtgtaatgccatttggtcggaagatggaaatatttacccagcaacc
atctcttcaattgatgctaaaaaagggacatgtatagttgtatacagcggatatgggaacagtgaggaacaaagtttggctgacctgcgctttccagata
cttctgaagcagaaagtgaccagagggagcaaggggcaggagctaaatggaaatgagcattcaacagatgaaagtgatagatcctcaagatcgcctcaat
gcaaagattctcaaaaccggggagataccaaagtcctcccagtgggaagggccagttccctcccggttccctcctccatttaagcctgggttttggaagg
cacggtggaaaatttggaataagccgcatcctttcctctctgggctggcccccaccatttctacctgggaccacctatggattccttcaaacctcctctc
aaagaacccctaaatgccttgttgaaaatg
3' EST: CF784255 [+]ttttttttttttttactaaagtaacaatattgcctaatatcacacattcatttttggaagcaatctaaatcattggatataacacaaagctagatggtga
cagttactttcagaatggtttcatatgtccagaagtggtttcttcatagcgcagaaacatggagcttctatgtaaacacccgacaccggctgttggacgt
ttcctattacttctggtgaggagattttccaaaggaagattccattcgcccttgctttagccccagatagtagccagtgtgatatccactcatgtaccaa
gcgatcagcatactgcccaatgcctcatcatcttcacaagcatcagggctcattggaggaggtggaggaatcataggtggtccaggtagaaatggtgggg
gccagccagagaggaaaggatgcgcttgatccaacttctcaccgtgcctttcaaacccaggcataaatggaggaggaacaggagggaaactggcccattc
ccactgcgaagacttttgggtattctccccgggtttttgagaaatccttttgcatttgaagggcgaatcctttgaagggaatcttaatccacccttgttc
catttcctggttttggaaagttggctttccaattccctaccccaaatctttaaaggtcctttctccgatggcccacccctgttggtagctttacccccgt
ttacgtttggggggtttgctcaactcttatttttttcccgtgtgggtcccatttttccccccagtaaaaaaaaaggtctttaacttccccctggggggga
aaaaaaaaaaaggccctgccccatcggggggggtttcccataaataccccccacaaaaaaaaccactttttttggggagtgttccccagcatccaaaaca
atggggggttatctccccccccctatataattcccccccccgccccgtggggtagattatccccaatacccttataattcccccatgtgtgttcccaccc
ctcttttttctcttttttaacacccataccacatatttttggaaaaagaaaaagaatgcgggattttgcccttgggtggatgtaaaaaatcattttttta
cccaatctcttttcccgngtaccataaaatggtgtccatttaacactcagttgtgtttgcacctaaaaattattcctacacttgttgtttaaagggagaa
gcgcgggctctcn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: