XB-CLONE-4352656
Clone Name: IMAGE:7027485
|
Gene:
sall1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784111 [+]
taattaatccccccccccccacctcttagagttcagcattcatgcccaatttgtcaaaagaaatttacaaatgctgttgtacttcaacagcatattagga
tgcatatgggagggcagattcctaatactcctattgctgaaagctatcctgactccatggaatcagatactgggtcctttgatgagaaaactattgatga
tctagacaatttctcagatgaaaacatggaagattgtccagacagcagtgtcccagacacccctaaatctgttgatgcatctcaagacagcctttcttct
tccccactgccgcctgaagtttcaagtatcactgctttagagaatcaaatgaaattaattaatgctggacttgctgaacagctccaagccagtctaaagt
caactgaaaatgggtctattgagggtgatggtatgaccaatgactcctcatctcttggtggtgacatggaaagccaaagtgctggaagccctgctgcctc
tgaatcgacttactccatgcatgctttgtctccttccaatagcacaaatgactatctgaaatcacccaacacagatgagaagattcatcgagctctgtct
cttgacccaaccagtggactgtcccctaccactgctaatggagggggctttggacctgacatctagcaacacagataaagtgatcaaagatgaactttta
ggtgggctattttcttttcccagaacgggggtaaatattaaaaaccctatatgtggaaatttgtg
3' EST: CF784110 [+]cattcccagcccttgtacctaaatccttctggaacatttcagggaacttaacagggtttcctccaagaaaagccataggtccatcaactgaaagccttct
tcctcttcttgcaggtgtgctgttccacatgtgagttcccatatgaaccttaaggttgccttttgtagtaaatgctcttccacatatagtgcaggcaaat
ggtttttcaccagtgtgagtccgctcatggatttgtaaagcactggaagaagagaatgatttcccacatgcattgcagtaatgctgcttaggagtccttc
tggccaaagcaggaagcagaactggcgatgtagcagaagatggcagagatcctgatgaaactatatttgtaggctgttctttaatgtcctgagaagagcc
atgcatgaaaccattaaattcagtctttattatagttgccagtgggttagtaggtgttgagggaactgttggggttggggtcatactggaactgggttca
aagagttgtgatggtagatccctcatctggtgagtgagcatatgctgtttcaaattgccctttgttgaaaagccacgattgcagactgtgcaaataaatg
gtctctctttggtatggcttctataatgaatgtccaaggcactctgacatgcaaaggttttgccacaaatatcacatatagtgtttttatatttaccccg
ttctcggaaaggaaatagcacaccctaaatgttcatcttttgatcactttaatccggtgttggcaaaatgtcaaagtccaaagcccccttcaattaacaa
aggggtaaggggaacaatccaccgggtttggggtcaaagagaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: