XB-CLONE-4353321
Clone Name: IMAGE:7026123
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Gene:
pnisr
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077655 [+]
CGGGCTTCCTCTCGGGCCCGGCTAGTCTCTGTGGTGGTGAGTTCCGCTGCTGAGCGCACAAGCGCCATATTGTTTGGAAGGGAGAGGAAAGATTCGGTGG
GATCGCTATAATATCTCCGGAGCTGATAGAGCAGAGGGCGGACCCTACGTTTGTGCTCGGCCCGTTGATAGCGATATATGTATATCTTTTGAGAGAATCT
AGCAGGGATGTGGGATCAAAGTGGACAACCATGGCAACAATGGCCGTTGAATCAGCAGCAGTGGATGGAGTCATTTCAGCATCGGCAGGATCCAAGCCAG
ATAGACTGGGCAGCTTTAGCACAAGCATGGATTGCTCAAAGAGAGGCAACAGGACAAGGAATAGTTGAGCAGCAAAATATAATGCCTAATGGGCAGGATC
TTCCACCAATGGAGCCAGGACCTAACAATCATGGCAGTTTTCAGGGAGATCCAAATTTTAATAGAATGTGGCAGCCTGAATGGGCAGGTATGCATCATCA
ACCACCTCCACATCACCCTCCGCCTGAACAGCCATGGATGCCTCCTGCCCCAGCTCCAATGGATGTTGTTCCTGTACCTGAGGACAGCAATAGCCAGGAC
AGTGGAGAGTTTACTGCTGAGAACAGACATATGTTTAACCAGAATAATCACAACTTTGGGGGGCCTCCTCCTGAAAACTTTTCAATGGGACCAGTAAATC
AATATGATTATCAGCATGGCTCAGGTTTTGGTCCTCCTCCAGGAGGATTTCACCCACCCTACTGGCAACAGGGACCACCTGGACCTCCAGGTCCCCCAGC
TCCACCACCAAACCGCAGGGAAAGGTCCTCTTTTAGAGAGAGGCAACGTTCACCTGTACCTATTCCTCCTAAGCAAGAACCTTTGCAGATTGATGCGGTT
AAGCGAAGAACGCTGCCAGCATGGATTCGTGAGGGACTTGAAAAGATGGAAAGGGAAAAGCAGAAGAAGTTGGAACGTGAAAGAATGGAACAGCAACGCT
CACAGAAAAAAAAAAAAAAAA
5' EST: CF783687 [+]taattaatccccccccccccccgggcttcctctcgggcccggctagtctctgtggtggtgagttccgctgctgagcgcacaagcgccatattgtttggaa
gggagaggaaagattcggtgggatcgctataatatctccggagctgatagagcagagggcggaccctacgtttgtgctcggcccgttgatagcgatatat
gtatatcttttgagagaatctagcagggatgtgggatcaaagtggacaaccatggcaacaatggccgttgaatcagcagcagtggatggagtcatttcag
catcggcaggatccaagccagatagactgggcagctttagcacaagcatggattgctcaaagagaggcaacaggacaaggaatagttgagcagcaaaata
taatgcctaatgggcaggatcttccaccaatggagccaggacctaacaatcatggcagttttcagggagatccaaattttaatagaatgtggcagcctga
atgggcaggtatgcatcatcaaccacctccacatcaccctccgcctgaacagccatggatgcctcctgccccagctccaatggatgttgttcctgtacct
gaggacagcaatagccaggacagtggagagtttactgctgagaacagacatatgttttacccgaataatcacaactttgggggggcctcctcctgaaaac
ttttttcatggggaccagtaaaatcaatatggattttcagccatggcctcagggttttgggtcttccctcccggaaggggattttccccccacccctt
3' EST: CF783688 [+]tttttttttttttttctgtgagcgttgctgttccattctttcacgttccaacttcttctgcttttccctttccatcttttcaagtccctcacgaatccat
gctggcagcgttcttcgcttaaccgcatcaatctgcaaaggttcttgcttaggaggaataggtacaggtgaacgttgcctctctctaaaagaggaccttt
ccctgcggtttggtggtggagctgggggacctggaggtccaggtggtccctgttgccagtagggtgggtgaaatcctcctggaggaggaccaaaacctga
gccatgctgataatcatattgatttactggtcccattgaaaagttttcaggaggaggccccccaaagttgtgattattctggttaaacatatgtctgttc
tcagcagtaaactctccactgtcctggctattgctgtcctcaggtacaggaacaacatccattggagctggggcaggaggcatccatggctgttcangcg
gagggtgatgtggaggtggttgatgatgcatacctgcccattcaggctgccacattctattaaaatttggatctccctgaaaactgccatgattgttaag
tcctggctccattggtggaagatcctgcccattaggcattatattttgctgctcaactattcccttgttcctgttgccctctcttttgagcaaatccatg
gcttgtggcctaaagcctgccccagtctaatctgggcttggaatccctggccgaatggctgaaaatgaactcccatcccactggccgtgcctgaaattca
acggggccatttgtttggccaatgggttggtcccaaccttttgaatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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