XB-CLONE-4354493
Clone Name: IMAGE:7026714
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Gene:
angel2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782957 [+]
taattaatcccccccccccccccggctgagctatggctgactttacgtggtgccgttgtagcgttgaggataaagccgctgactgtctgtgaggcgctgg
taaccacatgatcccatattatttggggaccctgagtagaagcagatttgtaggtgaccaacacacctatgagtagaaacttctttagatgtccatggtg
gacaggatattttccctggacccccaacctttccgtcagcagtttgaaccctcaagtaaattggaggccaagctattcgttgagaccaagcccagcttcc
tctcccaactcccttggtgttccctggagcccccaaccttcatgttggattcacgtctctaacctgaatatggcttctataagtgacgaaccgctaccga
aacgtaagagaacaagataggaagaccatgttgagcataatccaaaatcatccagtccctacagaggatatttgagtcccaccacgccatgcacctcctg
gggccccctccaaacaactaaaaattacacaagcagtcaaaaggggtccttttgcagaaagatggggaggactttcaatcctggattatctcagaaatta
ttcccaaaggggaacggtggggttttggtaaaggggatcccatctaagtttccagaagcggggcggcctatcgaccggaaggaaacttttgcccctatgg
gccccctctttggtcttaatgggcgatccttataaactggggttcccataagtatagncagatgactacgttgtagtgatttttccccaagataaaggca
tatttgttttcctccttgactatcatttgttttttggagtttggtgctaaaagcctctattcaagggggaggtcttataatcaatgtatctggggaaccg
gggtgtgatttaaattttgcgcgcgccctcaccaactcaagtgtgatatgatgaaataaaactctctattgttcatataaaaatgaaagttttgaggatg
tctatgttataattcgctttgtgatagtggatgaaaaaaaa
3' EST: CF782956 [+]ccaaacacacggttccatttggataattctgaataatacagattgaagtcttcccatcttctgctaatgacacctttgactgctgctgaatgtttagtgg
ttggagggggcccaggaggtgcatggagtggtgggactcgaatatcctttgtaggactggatgattttggcttctgctcaacatgttcttcctctcttgt
tctcttacgtttcggtagcggttcgtcacttttagaagccatattcaggttagagacgtgaatccaacatgaaggttgggggctccagggaacaccaagg
gagttgggagaggaagctgggcttggtctcaacgaatagcttggcctccaatttacttgagggttcaaactgctgacggaaaggttgggggtccagggaa
aatatcctgtccaccatggacatctaaagaagtttctacctcataggtgtgttggtcacctacaaaatctgcttctactcagggtccccaaataatatgg
gatcatgtggttaccagcgcctcacagacagtcagcggctttatccttcacgcctacaacggcaccacgttaaagtcagccatagcctcgcccggggggg
ggggggggggggattaattaactcccggtgcgaaaagaaaaaaatatctttttttcccgggccccggatagaaacccgaggttgccatttgggaccctgt
gggagtgggacccccttgttggtaaaaaacatgggaaaaatggggatgtttatttctatataggggccccctccaaaatcgtattggtggtttggatacc
ttgagggttaatggttcttaaatttggaagccgccttttctaaactatatga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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