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Summary Genes Attributions

Library Name: NIBB Mochii normalized Xenopus early gastrula library

Species: Xenopus laevis

Number Of Clones: 24006

Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).

Normalized: No Subtraction: No
Anatomy: whole organism Stage: NF stage 10.5

Vector DetailsVector Map
Name: pBluescript SK-

Type: phagemid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image
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