On Friday November 24, 2017 around 5 pm EST portions of Xenbase may be intermittently available.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Summary Genes Attributions
XB-LIB-173

Library Name: Yamamoto/Hyodo-Miura NIBB/NBRP Xenopus DMZ pCS2p+ cDNA library

Species: Xenopus laevis

Number Of Clones: 69183



Description: About 1000pieces of Keller explants were dissected at stage 10.5 and cultured in 0.1% BSA/1 x steinberg's solution. Total RNA was isolated by the acid guanidinium thiocyanate-phenolchloroform method from explants at stage 11~15. A cDNA library was made with the ZAP-cDNA Synthesis Kit (Stratagene ) according to manufacturer's instruction with minor modification. 4ug of polyA+RNA was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adaptor was ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end into the pCS2p+ vector. The average insert size was about 2kb. The host bacteria was XL2-Blue (Stratagene ).

Normalized: No Subtraction: No
Anatomy: Keller explant Stage: NF stage 10.5 to NF stage 15

Vector DetailsVector Map
Name: pBluescript SK-

Type: phagemid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

My Xenbase: [ Log-in / Register ]
version: [4.6.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556