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Summary Genes Attributions
XB-LIB-29

Library Name: NIH_XGC_tropNeu5

Species: Xenopus tropicalis

Number Of Clones: 2464



Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Normalized: No Subtraction: No
Anatomy: whole organism Stage: NF stage 14 to NF stage 18

Vector DetailsVector Map
Name: pCS22+

Type: plasmid

5' Restriction Site: PstI

3' Restriction Site: XhoI
Expression Image

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