Library Name: NIH_XGC_tropTe3
Species: Xenopus tropicalis
Number Of Clones: 11405
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe4 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.
|Normalized: No||Subtraction: No|
|Vector Details||Vector Map||Name: pCMV-SPORT6|
5' Restriction Site: SalI
3' Restriction Site: NotI