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Summary Genes Attributions
XB-LIB-50

Library Name: NIH_XGC_tropOvi1

Species: Xenopus tropicalis

Number Of Clones: 12184



Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).

Normalized: No Subtraction: No
Anatomy: oviduct Stage: frog

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

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