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Summary Genes Attributions

Library Name: NIH_XGC_tropMet10

Species: Xenopus tropicalis

Number Of Clones: 216

Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (0.5-1.5 kb fraction; NIH_XGC_tropMet9 contains the 1.5-2.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, not amplified. Library constructed at the DOE Joint Genome Institute.

Normalized: No Subtraction: No
Anatomy: whole organism Stage: NF stage 64

Vector DetailsVector Map

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI
Expression Image
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