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Summary Genes Attributions
XB-LIB-70

Library Name: NICHD_XGC_tropTail_m

Species: Xenopus tropicalis

Number Of Clones: 8663



Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.

Normalized: No Subtraction: No
Anatomy: tail Stage: NF stage 55 to NF stage 66

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: SalI

3' Restriction Site: NotI
Expression Image

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