|
XB-LIB-72
Library Name: NICHD_XGC_trop_25
|
Species: Xenopus tropicalis
|
Number Of Clones: 17121
|
Description: The library was made from dT primed cDNA and cloned into vector pCS107. PolyA RNA were primed with an oligo dT primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT -3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3') and digested with NotI. cDNA was size selected using 1.1% agarose gel electrophoresis (>0.6kb) then ligated into NotI and SalI digested pCS107 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute (Walnut Creek, CA) as part of the Xenopus Gene Collection project.
| Vector Details | Vector Map | Name: pCS107
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
|
[2.8.5]
