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XB-LIB-89
Library Name: Soares NXEG
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Species: Xenopus laevis
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Number Of Clones: 1957
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Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
| Vector Details | Vector Map | Name: pT7T3D-PacI
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI |  |
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[2.8.5]
