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Summary Genes Attributions
XB-LIB-89

Library Name: Soares NXEG

Species: Xenopus laevis

Number Of Clones: 1957



Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).

Normalized: No Subtraction: No
Anatomy: egg Stage: unfertilized egg stage

Vector DetailsVector Map
Name: pT7T3D-PacI

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: NotI
Expression Image

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