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Summary Genes Attributions
XB-LIB-99

Library Name: Xenopus laevis unfertilized egg cDNA library

Species: Xenopus laevis

Number Of Clones: 2072



Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. 500,000 unamplified cDNAs were mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine), also referred to as Xenopus_laevis_unfertilized_egg_cDNA_library.

Normalized: No Subtraction: No
Anatomy: egg Stage: unfertilized egg stage

Vector DetailsVector Map
Name: pBluescript SK-

Type: phagemid

5' Restriction Site: EcoRI

3' Restriction Site: XhoI
Expression Image

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