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GEO Series: GSE104848

Title: RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis

Summary

We combined cryosectining of oocytes along the animal-vegetal axis (first developmental axis) and RNA-Seq to determine localization profiles of coding and noncoding RNAs. It provides complete view on RNA localization. We found that nearly all RNAs are localized, but only small percentage is actively transported during oogenesis.


Contributors: Radek Šindelka, Radek Sindelka, Pavel Abaffy

Experiment Type: RNA profiles (mRNA, lncRNA, other RNA) were generated by deep sequencing, in biological triplicates (each oocyte divided into five segments), using Illumina HiSeq.

Article: XB-ART-54969, PubMed

Source: NCBI GEO, Xenbase Download

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
animal hemisphere - NF1 GSM2808769  GSM2808774  GSM2808779  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
animal hemisphere NF stage 1  
animal pole - NF1 GSM2808768  GSM2808773  GSM2808778  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
animal pole NF stage 1  
equatorial belt - NF1 GSM2808770  GSM2808775  GSM2808780  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
equatorial belt NF stage 1  
vegetal hemisphere - NF1 GSM2808771  GSM2808776  GSM2808781  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
vegetal hemisphere NF stage 1  
vegetal pole - NF1 GSM2808772  GSM2808777  GSM2808782  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
vegetal pole NF stage 1  
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Xla 

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