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GEO Series: GSE68972

Title: The identification of differentially expressed genes between animal and vegetal blastomeres in Xenopus laevis

Summary

To identify asymmetrically localized maternal mRNAs along the animal-vegetal axis in cleavage Xenopus embryos, we isolated animal and vegetal blastomeres at 8-cell stage, extracted the maternal mRNA respectively and analyzed them by RNA-seq technology. We identified 43 maternal transcripts significantly enriched in the vegetal region (FDR<0.05) by R/Bioconductor package DESeq


Contributors: Xiaopeng Ma, Guanni Sun, Zhirui Hu, Zheying Min, Xiaohua Yan, Zhenpo Guan, Hanxia Su, Yu Fu, YeGuang Chen, Michael Zhang, Qinghua Tao, Wei Wu

Experiment Type: RNAseq of animal and vegetal blastomeres with 2 biological replicates

Article: Xenbase, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
animal blastomere - NF4 GSM1689111  GSM1689112  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
animal blastomere NF stage 4 (8-cell)  
vegetal blastomere - NF4 GSM1689109  GSM1689110  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
vegetal blastomere NF stage 4 (8-cell)  
Select All 
Xtr   
Xla 

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