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GEO Series: GSE71006

Title: Cdx ParaHox genes acquired distinct developmental roles after gene duplication in vertebrate evolution

Summary

We examined the degree of regulatory and functional overlap between the three vertebrate Cdx genes using single and triple morpholino knock-down in Xenopus tropicalis followed by RNA-seq.


Contributors: Ferdinand Marl├ętaz, Harv Isaacs, Peter Holland

Experiment Type: Stage 14 (early neurula) embryos derived from eggs injected with morpholinos against Cdx1, Cdx2, Cdx4 and a mixture of all three plus corresponding uninjected embryos. All in triplicates.

Experiment Reagents: cdx1 MO2, cdx2 MO1, cdx4 MO1,

Article: Xenbase, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
WE - NF14 GSM1825055  GSM1825056  GSM1825057  RNA-Seq
Background: Xtr.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
   WE + Cdx2 MO - NF14 DEG GSM1825043  GSM1825044  GSM1825045  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection cdx2 MO1 cdx2 NF stage 1
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
   WE + Cdx4 MO - NF14 DEG GSM1825046  GSM1825047  GSM1825048  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection cdx4 MO1 cdx4 NF stage 1
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
   WE + cdx1 MO + Cdx2 MO + Cdx4 MO - NF14 DEG GSM1825049  GSM1825050  GSM1825051  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection cdx1 MO2 cdx1 NF stage 1 blastomere
morpholino injection cdx4 MO1 cdx4 NF stage 1 blastomere
morpholino injection cdx2 MO1 cdx2 NF stage 1 blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
   WE + cdx1 MO - NF14 DEG GSM1825040  GSM1825041  GSM1825042  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection cdx1 MO2 cdx1 NF stage 1
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
   WE + hbg1 MO - NF14 DEG GSM1825052  GSM1825053  GSM1825054  RNA-Seq
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection hbg1 cMO1 hbg1 NF stage 1
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 14  
Select All DEG      Select All 
Xtr   
Xla 

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