Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Return to search results

GEO Series: GSE76994

Title: Differential expression analysis of triple knockdown of TBP and TBP-related factors (TKD) in Xenopus laevis embryos at early developmental stage 10.5

Summary

We sequenced cDNA prepared from ribosomal RNA depleted total RNA of 10-10 embryos co-injected with TBP-,TBP2- and TLF-AS antisense oligonucleotides and with water to create expression profiles of triple knockdown of TBP/TBP-related factors (TKD) and control, respectively, in Xenopus laevis at early developmental stage 10.5. (according to Nieuwkoop and Faber). We executed differential expression analysis of sequence count data (DEseq).


Contributors: Gert Veenstra, E Gazdag, I Kruijsbergen, G Veenstra

Experiment Type: Differential expression analysis using RNA sequencing

Article: Xenbase, PubMed

Source: NCBI GEO, Xenbase FTP

Samples:
Sample View GSMs Assay Type
WE - NF10-10.5 GSM2042217  GSM2042218  RNA-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF10 to NF10.5  
   WE + tbp + tbpl2 + tbpl1 - NF10-10.5 DEG GSM2042215  GSM2042216  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
DMED injection tbp.L NF1 blastomere
DMED injection tbpl2.L NF1 blastomere
DMED injection tbpl1.L NF1 blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF10 to NF10.5  
Select All 
Xtr   
Xla 

Return to search results
Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.11.4


Major funding for Xenbase is provided by grant P41 HD064556