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GEO Series: GSE77363

Title: Genome-wide binding pattern of β-catenin during Xenopus gastrulation

Summary

The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.


Contributors: Richard Harland, Rachel Kjolby, Richard Harland

Experiment Type: Xenopus laevis emrbyos were injected with a triple FLAG tagged beta-catenin at levels that did not effect phenotype. Pools of about 500 embryos were fixed for ChIP processing. An input sample was taken prior to immunoprecipitation with a Flag antibody. Both input and ChIP material were used to make sequencing libraries.

Experiment Reagents: Tg(ctnnb1-FLAG).

Article: XB-ART-52028, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
input WE - NF11.5 GSM2050741  GSM2050742  GSM2050743  ChIP-Seq
Background: Xla.Outbred
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11.5  
   beta Catenin WE - NF11.5 GSM2050738  GSM2050739  GSM2050740  ChIP-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(ctnnb1-FLAG) ctnnb1.L NF stage 2 (2-cell) animal blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11.5 FLAG Ab1
Select All DEG      Select All 
Xtr   
Xla 

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