Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Return to search results

GEO Series: GSE83784

Title: Identification of microRNAs and microRNA targets in Xenopus ectoderm

Summary

Purpose: The establishment of cell lineages occurs via a dynamic progression of gene regulatory networks that underlie developmental commitment and differentiation. To investigate how microRNAs (miRs) function in this process, we compared miRs and miR targets at the initiation of the two major ectodermal lineages in Xenopus. Methods: We injected Xenopus laevis embryos with noggin or constitutively active BMP4 receptor (CABR) at two-cell stage and cut animal caps at stg. 8. Isolated animal caps were grown separately till mid gastrula and processed for either RNA isolation or immunoprecipitation. Results: We have identified over 400 miRNAs in early neural and epidermal ectoderm. The Ago-RNP RNAs from these tissues represent overlapping, yet distinct, subsets of genes. Moreover, the profile of Ago-RNP associated genes differs substantially from the profile of total RNAs in these tissues.


Contributors: Amy Sater, Vrutant Shah

Experiment Type: Identification of microRNAs and microRNA targets in establishment of early ectodermal tissues in Xenopus.

Experiment Reagents: nog.L, Tg(caHsa.bmpr1a).

Article: Xenbase, PubMed

Source: NCBI GEO, Xenbase FTP

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
myc animal cap - NF11 GSM2218816  GSM2218817  GSM2218818  RIP-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11 myc Ab10
   myc animal cap + CAbmpr1a - NF11 GSM2218808  GSM2218809  GSM2218810  GSM2218811  RIP-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(caHsa.bmpr1a) bmpr1a.L NF stage 2 (2-cell) animal blastomere
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11 myc Ab10
   myc animal cap + nog - NF11 GSM2218812  GSM2218813  GSM2218814  GSM2218815  RIP-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog.L nog.L NF stage 2 (2-cell) animal blastomere
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11 myc Ab10
WE + CAbmpr1a - NF11 GSM2218819  GSM2218820  GSM2218821  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(caHsa.bmpr1a) bmpr1a.L NF stage 2 (2-cell) animal blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11  
WE + nog - NF11 GSM2218822  GSM2218823  GSM2218824  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog.L nog.L NF stage 2 (2-cell) animal blastomere
Conditions:
Tissue Assay Stages ChIP Antibody
embryo NF stage 11  
animal cap + CAbmpr1a - NF11 GSM2218802  GSM2218803  GSM2218804  miRNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(caHsa.bmpr1a) bmpr1a.L NF stage 2 (2-cell) animal blastomere
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11  
animal cap + nog - NF11 GSM2218805  GSM2218806  GSM2218807  miRNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection nog.L nog.L NF stage 2 (2-cell) animal blastomere
explant   NF stage 8 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11  
Select All DEG      Select All 
Xtr   
Xla 

Return to search results
Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.15.0
Major funding for Xenbase is provided by grant P41 HD064556