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XPax-2: mid-hindbrain junction, presumptive pronephros, rectal diverticulum, pharyngeal aches, otic vesicle and spinal chord.
Although sequence homology indicates that this gene is a Pax-2 orthologue, it is expressed in the thyroid gland, normally a site of Pax-8 expressionin mammals. In mice the thyroid is not effected by Pax-2 disruption, but it is by Pax-8 disruption. Conversely, the Xenopus gene most closely related to mammalian Pax-8, XPax-8, is not expressed in the thyroid gland. Carroll and Vize have shown that XPax-8 and XPax-2 are functionally redundant, and it appears that these orthologues have retained different promoter elements in different taxa.
Click on images to view enlargements (6K to 7K, < 2 seconds).
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| Stage 12.5 embryo. White arrow indicates one of two neural patches of XPax-2 expression. Staining corresponds to the future mid-hindbrain junction. | Stage 17. Black arrow indicates eye field expression. Black double arrow indicates blastopore expression. | Stage 20. Yellow arrow indicates future otic vesicle expression. Red double arrow indicates pronephric anlage expression. | Stage 29. Red arrow indicates pharyngeal arch staining. White arrowhead indicates pronephric duct expression. Yellow arrow indicates spinal cord staining. Note periodic pattern. | Stage 35/36. Red arrowhead indicates thyroid gland expression. White arrow indicates increased expression in the nephrostomes of the nephric tubules. |
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| Stage 33/34. Ventral view of thyroid gland. | Stage 33/34. Close-up of posterior end of tadpole. Black arrow indicates lack of expression between non-fused posteriorly migrating nephric duct and anteriorly migrating rectal diverticula. | Stage 35/36. Black arrow indicates region of increased XPax-2 expression where duct and rectal diverticula are fusing. |
Anterior is to the left in all panels. Dorsal is up in panels C,D,E,G and H. A and B are dorsal views, C, D, E and H are lateral views and F is a ventral view.
| Cloned by: | Original description: | ||
| Tom Carroll, also see Heller et al. | Carroll, T.J. and Vize, P.D. (1999) Synergism between Pax-8 and lim-1 in embryonic kidney development. Dev Biol. 214: 46-59. | ||
| Images submitted by: | Sequence | ||
| Peter Vize | AF179300 | ||
| Probe available from: | Other papers by: | ||
| Peter Vize | Tom Carrol and Peter Vize | ||
| In situ probe details: | Other papers on: | ||
| plasmid name: | antisense RE cut: | antisense transcription: | XPax genes |
| mRNA generation | |||
| plasmid name: | sense RE cut: | sense transcription: | |
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