p63: sensorial epidermis and neural crest. To view the restriction to the sensorial layer of the epidermis view the confocal section stained with 4A4.

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mRNA in situ hybridization (Xp63)		protein immunohistochemistry (4A4; anti-p63)
stage 17	stage 17, dorsal			stage 17 anterior	stage 17, dorsal
the crescent of expression at the anterior-most end of the neural plate probably corresponds to the stomodeal-hypophyseal anlage. Within the epidermis staining is very even with the exception of an arc ventral to the presumptive hindbrain region of the neural plate which is devoid of Xp63 RNA. This region overlies the future branchial arches				Wholemount immunohistochemistry with antibody 4A4 (Yang et al. 1998) detects a nuclear protein whose distribution is very similar to that of Xp63 mRNA (see left)
stage 20 - 21 anterior	stage 20 - 21 dorsal.			stage 19	stage 19 dorsal
expression in some of the forming cranial neural crest, in addition to continued expression in the stomodeal-hypophyseal anlage and in most of the epidermis
stage 24 anterior	stage 24 dorsal			stage 23-24 anterior	stage 23 - 24 dorsal
expression observed in some of the forming cranial neural crest, in addition to continued expression in the stomodeal-hypophyseal anlage and in most of the epidermis. Within the cranial crest expression of Xp63 is observed in the mandibular and branchial crest, but is clearly absent from the hyoid crest.				4A4 immunoreactivity remains particularly high in majority of the epidermis, but consistent with the distribution of Xp63 RNA, is never expressed in the branchial placode ectoderm
red indicates expression in crest drived fin, stage 31+	stage 34 lateral. expression in branchial arches and tail fin			stage 31 anterior	stage 28 lateral
stage 24	stage 30			stage 35-36	stage 38
As the lens begins to form from the deeper layer of sensorial ectoderm (Nieuwkoop and Faber, 1994) Xp63 expression in this layer is extinguished. Concomitant with the internalization of the lens, expression of Xp63 returns except for a small patch directly above the lens. By stage 38 even this small patch has reacquired Xp63 expression. Xp63 protein is also absent from the other forming ectodermal placodes such as the dorso-lateral and olfactory placodes.

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