4A6: Anti-pronephric distal tubule and pronephric duct monoclonal antibody, IgG subtype. Available as culture supernatent, use diluted 1:5 with blocking buffer.

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A.	B.	C.	D.
Stage 38-39, lateral view. The distal segment of the pronephric tubules is solidly stained. The more caudal duct is stained in a mosaic fashion.	Close up of stage 38-39 staining. The three nephrostomes are also stained.	Mosaic expression in the pronephric duct	Isolated 4A6 positive cells in the rectal diverticulum/cloacal portion of the duct
E	F	G	H
Stage 38 -39 embryo double stained with 3G8 in blue and 4A6 in red. The two antigens are present in distinct domains within the tubules.	dorsal view of 4A6 positive tissues	stage 42 lateral staining	wax section of duct stained with 4A6

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