A.
stg 4
B.
stg 10
C.
stg 10.25
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geminin: early, strong neural marker. Staining throughout the animal hemisphere during blastula stages but localizes to the presumptive neural territory by st. 10+. Also an early marker of the sensory placodes.
Click on images to view enlargements (6K to 7K, < 2 seconds). These images have been previously published. Copyright © to images resides with the Company of Biologists, 1998.
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A.
stg 4
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B.
stg 10
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C.
stg 10.25
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D. stg 10.25 |
| geminin-purple | geminin-purple | geminin-pink / brachyury-aqua | geminin-pink / brachyury-aqua |
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| E. stg 12.5 | F. stg 12 | G. stg 13.5 | H |
| geminin-purple | geminin-pink / BMP4-aqua | geminin-purple | geminin-pink / XDelta-1-aqua |
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| I. stg 20 | J. stg 38 | K. stg 38 | |
| geminin-purple | geminin-purple | geminin-purple |
Geminin staining is purple in singly stained embryos. (C, D, F, H) Geminin expression is pink in doubly stained embryos. The expression patterns stained in aqua are brachyury (C, D), BMP4 (F), or X-Delta-1 (H). The original clone was isolated in an expression screen of a st. 14-20 neurula cDNA library.
| Cloned by: | Original description: | ||
| Kris Kroll et al. |
Kroll, Kristen L., Salic, Adrian N., Evans, Louis M., and Kirschner, Marc W. 1998. Geminin, a neuralizing molecule that demarcates the future neural plate at the onset of gastrulation. Development 125, 3247-3258. |
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| Images submitted by: | Sequence | ||
| Kris Kroll | Genbank AF067856 | ||
| Probe available from: | Other papers by: | ||
| Kris Kroll | Kris Kroll and Marc Kirschner | ||
| In situ probe details: EcoRI - XhoI insert in CS2+ | Other papers on: | ||
| plasmid name: | antisense RE cut: | antisense transcription: | Xenopus geminin |
| CS2+geminin | EcoRI | T3 | |
| mRNA generation | RT-PCR primers | ||
| plasmid name: | sense RE cut: | sense transcription: | + 5'-gctggacatgtacagtacatg-3' |
| NotI | SP6 | - 5'-atcacctcacataaaggctgg-3' | |
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