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In the present paper we report the cloning and sequencing of the cDNA encoding two calreticulin isoforms from Xenopus laevis central nervous system. The two isoforms display 93% identity at the amino acid level. The predicted amino acid sequences of the amphibian calreticulins are very similar (76%) to those of mammalian liver and skeletal muscle. Xenopus laevis calreticulins are characterized by a very acidic c-terminal domain endowed with the endoplasmic-reticulum retention signal KDEL. The cDNAs of both clones encode an N-glycosylation consensus sequence. A third clone of calreticulin was also identified. The restriction map of this clone was clearly distinct from that of the two sequenced clones. These results indicate the existence of multiple calreticulin isoforms in the central nervous system and open questions about their functional role in different cells and/or subcellular compartments.
Baksh,
Expression of calreticulin in Escherichia coli and identification of its Ca2+ binding domains.
1991, Pubmed
Baksh,
Expression of calreticulin in Escherichia coli and identification of its Ca2+ binding domains.
1991,
Pubmed Burgoyne,
Locating intracellular calcium stores.
1991,
Pubmed Burk,
cDNA cloning, functional expression, and mRNA tissue distribution of a third organellar Ca2+ pump.
1989,
Pubmed Carafoli,
Calcium pump of the plasma membrane.
1991,
Pubmed Eggermont,
Evidence for two isoforms of the endoplasmic-reticulum Ca2+ pump in pig smooth muscle.
1989,
Pubmed Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed Fliegel,
Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum.
1989,
Pubmed Fliegel,
Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing.
1987,
Pubmed Furuichi,
Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400.
1989,
Pubmed Henne,
Inositol 1,4,5-trisphosphate and 5'-GTP induce calcium release from different intracellular pools.
1987,
Pubmed Khanna,
Calregulin: purification, cellular localization, and tissue distribution.
1987,
Pubmed MacLennan,
Isolation of a calcium-sequestering protein from sarcoplasmic reticulum.
1971,
Pubmed McPherson,
Solubilization and biochemical characterization of the high affinity [3H]ryanodine receptor from rabbit brain membranes.
1990,
Pubmed Meldolesi,
Intracellular Ca2+ storage organelles in non-muscle cells: heterogeneity and functional assignment.
1990,
Pubmed Milner,
Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum.
1991,
Pubmed Munro,
A C-terminal signal prevents secretion of luminal ER proteins.
1987,
Pubmed Nakai,
Primary structure and functional expression from cDNA of the cardiac ryanodine receptor/calcium release channel.
1990,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Smith,
Multiple zones in the sequence of calreticulin (CRP55, calregulin, HACBP), a major calcium binding ER/SR protein.
1989,
Pubmed Südhof,
Structure of a novel InsP3 receptor.
1991,
Pubmed Treves,
Molecular cloning, functional expression and tissue distribution of the cDNA encoding frog skeletal muscle calsequestrin.
1992,
Pubmed Treves,
Calreticulin is a candidate for a calsequestrin-like function in Ca2(+)-storage compartments (calciosomes) of liver and brain.
1990,
Pubmed Trevesø,
Frog brain expresses a 60 KDa Ca2+ binding protein similar to mammalian calreticulin.
1991,
Pubmed Van,
Four intracisternal calcium-binding glycoproteins from rat liver microsomes with high affinity for calcium. No indication for calsequestrin-like proteins in inositol 1,4,5-trisphosphate-sensitive calcium sequestering rat liver vesicles.
1989,
Pubmed Volpe,
Calsequestrin, a component of the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of chicken cerebellum.
1990,
Pubmed Volpe,
Heterogeneity of microsomal Ca2+ stores in chicken Purkinje neurons.
1991,
Pubmed von Heijne,
Signal sequences. The limits of variation.
1985,
Pubmed Walton,
Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons.
1991,
Pubmed Young,
Efficient isolation of genes by using antibody probes.
1983,
Pubmed
