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The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.
Chatterton,
Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.
2002, Pubmed,
Xenbase
Chatterton,
Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.
2002,
Pubmed
,
Xenbase Das,
Increased NMDA current and spine density in mice lacking the NMDA receptor subunit NR3A.
1998,
Pubmed
,
Xenbase Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed Furukawa,
Subunit arrangement and function in NMDA receptors.
2005,
Pubmed Garcia-Parajo,
The nature of fluorescence emission in the red fluorescent protein DsRed, revealed by single-molecule detection.
2001,
Pubmed He,
Constraining the subunit order of rod cyclic nucleotide-gated channels reveals a diagonal arrangement of like subunits.
2000,
Pubmed
,
Xenbase HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed Laube,
Evidence for a tetrameric structure of recombinant NMDA receptors.
1998,
Pubmed
,
Xenbase Leake,
Stoichiometry and turnover in single, functioning membrane protein complexes.
2006,
Pubmed Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase MacKinnon,
Functional stoichiometry of Shaker potassium channel inactivation.
1993,
Pubmed
,
Xenbase Premkumar,
Stoichiometry of recombinant N-methyl-D-aspartate receptor channels inferred from single-channel current patterns.
1997,
Pubmed
,
Xenbase Sonnleitner,
Structural rearrangements in single ion channels detected optically in living cells.
2002,
Pubmed
,
Xenbase Young,
Distinct structural determinants of efficacy and sensitivity in the ligand-binding domain of cyclic nucleotide-gated channels.
2004,
Pubmed
,
Xenbase Zheng,
Rod cyclic nucleotide-gated channels have a stoichiometry of three CNGA1 subunits and one CNGB1 subunit.
2002,
Pubmed
,
Xenbase Zhong,
The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.
2002,
Pubmed
