Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-10576
Biochem Biophys Res Commun 2000 Aug 11;2743:852-8. doi: 10.1006/bbrc.2000.3240.
Show Gene links Show Anatomy links

Expression cloning of KCRF, a potassium channel regulatory factor.

Keren-Raifman T , Ivanina T , Bismuth Y , Dascal N .


???displayArticle.abstract???
By functional coexpression screening of a rat cDNA library in Xenopus oocytes, we have cloned a protein (KCRF: K Channel Regulatory Factor) that reduces currents of several K(+) channels: G protein-activated GIRK1/4 (K(ir)3.1/K(ir)3.4), inward rectifier IRK1 (K(ir)2.1), and voltage-dependent K(V)1.1/K(V)beta1.1. KCRF did not modulate two other K(+) channels: ROMK1 (K(ir)1.1) and GIRK1/2 (K(ir)3.1/K(ir)3.2) and the voltage-dependent L-type Ca(2+) channels. Western blot analysis showed that KCRF is ubiquitous in rat tissues. Biochemical and electrophysiological experiments revealed that coexpression of KCRF causes a decrease in the level of expression of IRK1 and K(V)1.1/K(V)beta1.1 proteins in the oocytes.

???displayArticle.pubmedLink??? 10924366
???displayArticle.link??? Biochem Biophys Res Commun
???displayArticle.grants??? [+]

Species referenced: Xenopus
Genes referenced: kcna1 kcnj1 kcnj12 kcnj2 kcnj3