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Vacuole formation in fatigued skeletal muscle fibres from frog and mouse: effects of extracellular lactate.
Lännergren J
,
Bruton JD
,
Westerblad H
.
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Isolated, living muscle fibres from either Xenopus or mouse were observed in a confocal microscope and t-tubules were visualized with sulforhodamine B. Observations were made before and after fatiguing stimulation. In addition, experiments were performed on fibres observed in an ordinary light microscope with dark-field illumination. In Xenopus fibres, recovering after fatigue, t-tubules started to show dilatations 2-5 min post-fatigue. These swellings increased in size over the next 10-20 min to form vacuoles. After 2-3 h of recovery the appearance of the fibres was again normal and force production, which had been markedly depressed 10-40 min post-fatigue, was close to control. Vacuoles were not observed in mouse fibres, fatigued with the same protocol and allowed to recover. In Xenopus fibres, fatigued in normal Ringer solution and allowed to recover in Ringer solution with 30-50 mM L-lactate substituting for chloride (lactate-Ringer), the number and size of vacuoles were markedly reduced. Also, force recovery was significantly faster. Replacement of chloride by methyl sulphate or glucuronate had no effect on vacuolation. Resting Xenopus fibres exposed to 50 mM lactate-Ringer and transferred to normal Ringer solution displayed vacuoles within 5-10 min, but to a smaller extent than after fatigue. Vacuolation was not associated with marked force reduction. Mouse fibres, fatigued in 50 mM lactate-Tyrode (L-lactate substituting for chloride in Tyrode solution) and recovering in normal Tyrode solution, displayed vacuoles for a limited period post-fatigue. Vacuolation had no effect on force production. The results are consistent with the view that lactate, formed during fatigue, is transported into the t-tubules where it attracts water and causes t-tubule swelling and vacuolation. This vacuolation may be counteracted in vivo due to a gradual extracellular accumulation of lactate during fatigue.
Bruton,
Mechano-sensitive linkage in excitation-contraction coupling in frog skeletal muscle.
1995, Pubmed,
Xenbase
Bruton,
Mechano-sensitive linkage in excitation-contraction coupling in frog skeletal muscle.
1995,
Pubmed
,
Xenbase
Bruton,
Effects of CO2-induced acidification on the fatigue resistance of single mouse muscle fibers at 28 degrees C.
1998,
Pubmed
Bruton,
Effects of repetitive tetanic stimulation at long intervals on excitation-contraction coupling in frog skeletal muscle.
1996,
Pubmed
,
Xenbase
Chin,
The role of elevations in intracellular [Ca2+] in the development of low frequency fatigue in mouse single muscle fibres.
1996,
Pubmed
Dutka,
Effect of lactate on depolarization-induced Ca(2+) release in mechanically skinned skeletal muscle fibers.
2000,
Pubmed
Endo,
Entry of fluorescent dyes into the sarcotubular system of the frog muscle.
1966,
Pubmed
Favero,
Lactate inhibits Ca(2+) -activated Ca(2+)-channel activity from skeletal muscle sarcoplasmic reticulum.
1997,
Pubmed
Gallagher,
Osmotic 'detubulation' in frog muscle arises from a reversible vacuolation process.
1997,
Pubmed
Gonzalez-Serratos,
Composition of vacuoles and sarcoplasmic reticulum in fatigued muscle: electron probe analysis.
1978,
Pubmed
HUTTER,
The chloride conductance of frog skeletal muscle.
1960,
Pubmed
Halestrap,
The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors.
1975,
Pubmed
Horn,
In vitro and in vivo ultrastructural changes induced by macrolide antibiotic LY281389.
1996,
Pubmed
Juel,
Lactate transport in skeletal muscle - role and regulation of the monocarboxylate transporter.
1999,
Pubmed
Juel,
Lactate-proton cotransport in skeletal muscle.
1997,
Pubmed
Jóhannsson,
Cellular and subcellular expression of the monocarboxylate transporter MCT1 in rat heart. A high-resolution immunogold analysis.
1997,
Pubmed
Krolenko,
Reversible vacuolation of the transverse tubules of frog skeletal muscle: a confocal fluorescence microscopy study.
1995,
Pubmed
Lamb,
Raised intracellular [Ca2+] abolishes excitation-contraction coupling in skeletal muscle fibres of rat and toad.
1995,
Pubmed
Libelius,
T-tubule endocytosis in dystrophic chicken muscle and its relation to muscle fiber degeneration.
1979,
Pubmed
Lännergren,
Vacuole formation in fatigued single muscle fibres from frog and mouse.
1999,
Pubmed
,
Xenbase
Lännergren,
Slow recovery of force in single skeletal muscle fibres.
1996,
Pubmed
Lännergren,
The effect of temperature and stimulation scheme on fatigue and recovery in Xenopus muscle fibres.
1988,
Pubmed
,
Xenbase
Lännergren,
Transient appearance of vacuoles in fatigued Xenopus muscle fibres.
1990,
Pubmed
,
Xenbase
Malouf,
Proliferation of the surface-connected intracytoplasmic membranous network in skeletal muscle disease.
1986,
Pubmed
Mason,
A microelectrode study of the mechanisms of L-lactate entry into and release from frog sartorius muscle.
1988,
Pubmed
Nagesser,
Metabolic changes with fatigue in different types of single muscle fibres of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Nik-Zainal,
Cardiac glycosides inhibit detubulation in amphibian skeletal muscle fibres exposed to osmotic shock.
1999,
Pubmed
Thompson,
Muscle fatigue in the frog semitendinosus: role of the high-energy phosphates and Pi.
1992,
Pubmed
Vaughan-Jones,
Non-passive chloride distribution in mammalian heart muscle: micro-electrode measurement of the intracellular chloride activity.
1979,
Pubmed
Westerblad,
Myoplasmic free Mg2+ concentration during repetitive stimulation of single fibres from mouse skeletal muscle.
1992,
Pubmed
Westerblad,
Force and membrane potential during and after fatiguing, intermittent tetanic stimulation of single Xenopus muscle fibres.
1986,
Pubmed
,
Xenbase
Westerblad,
The relation between force and intracellular pH in fatigued, single Xenopus muscle fibres.
1988,
Pubmed
,
Xenbase
Westerblad,
Reversible increase in light scattering during recovery from fatigue in Xenopus muscle fibres.
1990,
Pubmed
,
Xenbase
Westerblad,
Changes of intracellular pH due to repetitive stimulation of single fibres from mouse skeletal muscle.
1992,
Pubmed
