Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2000 Aug 01;1915:3918-31. doi: 10.1093/emboj/19.15.3918.
Show Gene links
Show Anatomy links
Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.
???displayArticle.abstract???
In cell-free extracts of Xenopus eggs that support the assembly of replication-competent nuclei, we found that lamin B(3) specifically associates with four polypeptides (termed SLAPs, soluble lamin associated proteins). Here, one SLAP is identified as the nuclear pore complex protein Nup153, one member of the F/GXFG motif-containing nucleoporins. In vitro translated Nup153 and lamin B(3) co-immunoprecipitate, and lamin B(3) interacts specifically with the C-terminal domain of Nup153. During nuclear envelope assembly, other F/GXFG-containing nucleoporins are incorporated into the nuclear envelope preceding lamina assembly. Incorporation of Nup153 occurs at the same time as lamina assembly. When lamina assembly is prevented using the dominant-negative mutant XlaminB delta 2+, Nup153 does not appear at the nuclear envelope, while other F/GXFG-containing nucleoporins and Nup93 are recruited normally. When the lamina of pre-assembled nuclei is disrupted using the same dominant-negative mutant, the distribution of other nucleoporins is unaffected. However, Nup153 recruitment at the nuclear envelope is lost. Our results indicate that both the recruitment and maintenance of Nup153 at the pore are dependent upon the integrity of the lamina.
Akey,
Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy.
1993, Pubmed,
Xenbase
Akey,
Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy.
1993,
Pubmed
,
Xenbase
Alessi,
Mechanism of activation of protein kinase B by insulin and IGF-1.
1996,
Pubmed
Aris,
Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins.
1989,
Pubmed
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Bodoor,
Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.
1999,
Pubmed
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Cordes,
Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments.
1997,
Pubmed
,
Xenbase
Delphin,
RanGTP targets p97 to RanBP2, a filamentous protein localized at the cytoplasmic periphery of the nuclear pore complex.
1997,
Pubmed
Drummond,
Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.
1999,
Pubmed
,
Xenbase
Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed
Dyer,
Cell cycle changes in A-type lamin associations detected in human dermal fibroblasts using monoclonal antibodies.
1997,
Pubmed
Ellis,
Aberrant intracellular targeting and cell cycle-dependent phosphorylation of emerin contribute to the Emery-Dreifuss muscular dystrophy phenotype.
1998,
Pubmed
Ellis,
GST-lamin fusion proteins act as dominant negative mutants in Xenopus egg extract and reveal the function of the lamina in DNA replication.
1997,
Pubmed
,
Xenbase
Enarson,
Amino-terminal sequences that direct nucleoporin nup153 to the inner surface of the nuclear envelope.
1998,
Pubmed
Favreau,
Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210.
1996,
Pubmed
Firmbach-Kraft,
Analysis of nuclear lamin isoprenylation in Xenopus oocytes: isoprenylation of lamin B3 precedes its uptake into the nucleus.
1995,
Pubmed
,
Xenbase
Goldberg,
Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.
1997,
Pubmed
,
Xenbase
Goldberg,
Xenopus lamin B3 has a direct role in the assembly of a replication competent nucleus: evidence from cell-free egg extracts.
1995,
Pubmed
,
Xenbase
Goldberg,
The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.
1996,
Pubmed
,
Xenbase
Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase
Haraguchi,
Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes.
2000,
Pubmed
Hutchison,
Periodic DNA synthesis in cell-free extracts of Xenopus eggs.
1987,
Pubmed
,
Xenbase
Hutchison,
The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro.
1988,
Pubmed
,
Xenbase
Jenkins,
Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
1993,
Pubmed
,
Xenbase
Jones,
Activation of the Xenopus cyclin degradation machinery by full-length cyclin A.
1996,
Pubmed
,
Xenbase
Kill,
The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts.
1991,
Pubmed
,
Xenbase
Kiseleva,
Active nuclear pore complexes in Chironomus: visualization of transporter configurations related to mRNP export.
1998,
Pubmed
Kiseleva,
RNP export is mediated by structural reorganization of the nuclear pore basket.
1996,
Pubmed
Meier,
The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs.
1991,
Pubmed
,
Xenbase
Melchior,
Mechanisms of nuclear protein import.
1995,
Pubmed
Moir,
Expression in Escherichia coli of human lamins A and C: influence of head and tail domains on assembly properties and paracrystal formation.
1991,
Pubmed
Nakielny,
Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain.
1999,
Pubmed
Newport,
A lamin-independent pathway for nuclear envelope assembly.
1990,
Pubmed
,
Xenbase
Panté,
The nuclear pore complex.
1993,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Panté,
Toward the molecular details of the nuclear pore complex.
1994,
Pubmed
Panté,
Sequential binding of import ligands to distinct nucleopore regions during their nuclear import.
1996,
Pubmed
,
Xenbase
Radu,
Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.
1995,
Pubmed
,
Xenbase
Rutherford,
Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.
1997,
Pubmed
,
Xenbase
Shah,
Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.
1998,
Pubmed
,
Xenbase
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Stick,
Changes in the nuclear lamina composition during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Sukegawa,
A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.
1993,
Pubmed
Theodoropoulos,
PBC68: a nuclear pore complex protein that associates reversibly with the mitotic spindle.
1999,
Pubmed
Ullman,
The nucleoporin nup153 plays a critical role in multiple types of nuclear export.
1999,
Pubmed
,
Xenbase
Vorburger,
Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.
1989,
Pubmed
Waseem,
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
1990,
Pubmed
Wiese,
Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport-dependent 'envelope smoothing' event.
1997,
Pubmed
,
Xenbase
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yang,
Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.
1997,
Pubmed
Zhang,
Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract.
1996,
Pubmed
,
Xenbase
