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Expression of human hyaluronan synthases in response to external stimuli.
Jacobson A
,
Brinck J
,
Briskin MJ
,
Spicer AP
,
Heldin P
.
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In the present study we have investigated the expression of mRNAs for hyaluronan synthase isoforms (HAS1, HAS2 and HAS3) in different cells in response to various stimuli. Human mesothelial cells, which synthesize large amounts of hyaluronan, express mRNAs encoding all three HAS isoforms, whereas their transformed counterparts, mesothelioma cells, which produce only minute amounts of hyaluronan, express only HAS3 mRNA. Human lung fibroblasts and the glioma cell line U-118 MG express only the HAS2 and HAS3 genes. The expression of the transcripts was higher in subconfluent than in confluent cultures and was well correlated with the production of hyaluronan by the cells. Stimulation of mesothelial cells with platelet-derived growth factor-BB induced an up-regulation of mRNA for HAS2 to a maximum after 6 h of stimulation; HAS1 and HAS3 genes were only induced slightly. Transforming growth factor-beta1 reduced HAS2 mRNA slightly, and hydrocortisone reduced it strongly, within 6 h of stimulation in mesothelial cell cultures but did not significantly affect the expression of mRNAs for HAS1 and HAS3. Induction of HAS1 and HAS2 protein levels in response to the stimuli above correlated with HAS transcript levels. Thus the expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli suggesting distinct functional roles of the three proteins.
Asplund,
Human mesothelioma cells produce factors that stimulate the production of hyaluronan by mesothelial cells and fibroblasts.
1993, Pubmed
Asplund,
Human mesothelioma cells produce factors that stimulate the production of hyaluronan by mesothelial cells and fibroblasts.
1993,
Pubmed
Asplund,
Hyaluronan receptors are expressed on human malignant mesothelioma cells but not on normal mesothelial cells.
1994,
Pubmed
Asplund,
Characterization of hyaluronan synthase from a human glioma cell line.
1998,
Pubmed
,
Xenbase
Becq,
Phosphatase inhibitors activate normal and defective CFTR chloride channels.
1994,
Pubmed
Brinck,
Expression of recombinant hyaluronan synthase (HAS) isoforms in CHO cells reduces cell migration and cell surface CD44.
1999,
Pubmed
,
Xenbase
DeAngelis,
Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes.
1993,
Pubmed
,
Xenbase
DeAngelis,
Yeast-derived recombinant DG42 protein of Xenopus can synthesize hyaluronan in vitro.
1996,
Pubmed
,
Xenbase
DeAngelis,
Hyaluronan synthase of chlorella virus PBCV-1.
1997,
Pubmed
,
Xenbase
DeAngelis,
Identification and molecular cloning of a unique hyaluronan synthase from Pasteurella multocida.
1998,
Pubmed
,
Xenbase
Heldin,
Effect of growth factors on hyaluronan synthesis in cultured human fibroblasts.
1989,
Pubmed
Heldin,
Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells.
1992,
Pubmed
,
Xenbase
Honda,
Cooperative enhancement of hyaluronic acid synthesis by combined use of IGF-I and EGF, and inhibition by tyrosine kinase inhibitor genistein, in cultured mesothelial cells from rabbit pericardial cavity.
1991,
Pubmed
,
Xenbase
Itano,
Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Klewes,
Intracellular signal transduction for serum activation of the hyaluronan synthase in eukaryotic cell lines.
1994,
Pubmed
,
Xenbase
Knudson,
Hyaluronan-binding proteins in development, tissue homeostasis, and disease.
1993,
Pubmed
Laurent,
Hyaluronan.
1992,
Pubmed
Myers,
Effects of synovial fluid hyaluronan concentration and molecular size on clearance of protein from the canine knee.
1995,
Pubmed
Roboz,
Hyaluronic acid content of effusions as a diagnostic aid for malignant mesothelioma.
1985,
Pubmed
Rooney,
The role of hyaluronan in tumour neovascularization (review).
1995,
Pubmed
Sherman,
Hyaluronate receptors: key players in growth, differentiation, migration and tumor progression.
1994,
Pubmed
Shyjan,
Functional cloning of the cDNA for a human hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Spicer,
Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase.
1997,
Pubmed
,
Xenbase
Spicer,
Molecular cloning and characterization of a putative mouse hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Spicer,
Chromosomal localization of the human and mouse hyaluronan synthase genes.
1997,
Pubmed
,
Xenbase
Spicer,
Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family.
1998,
Pubmed
,
Xenbase
Sugiyama,
Putative hyaluronan synthase mRNA are expressed in mouse skin and TGF-beta upregulates their expression in cultured human skin cells.
1998,
Pubmed
,
Xenbase
Suzuki,
Stimulation of hyaluronan biosynthesis by platelet-derived growth factor-BB and transforming growth factor-beta 1 involves activation of protein kinase C.
1995,
Pubmed
Tengblad,
Quantitative analysis of hyaluronate in nanogram amounts.
1980,
Pubmed
Tirone,
Hyaluronan synthesis by mouse cumulus cells is regulated by interactions between follicle-stimulating hormone (or epidermal growth factor) and a soluble oocyte factor (or transforming growth factor beta1).
1997,
Pubmed
Toole,
Hyaluronan and its binding proteins, the hyaladherins.
1990,
Pubmed
Watanabe,
Molecular identification of a putative human hyaluronan synthase.
1996,
Pubmed
,
Xenbase
Weigel,
Hyaluronan synthases.
1997,
Pubmed
,
Xenbase
Wells,
Correlation between increased hyaluronan localized in arthritic synovium and the presence of proliferating cells. A role for macrophage-derived factors.
1992,
Pubmed
West,
Angiogenesis induced by degradation products of hyaluronic acid.
1985,
Pubmed
Yamada,
The gene structure and promoter sequence of mouse hyaluronan synthase 1.
1998,
Pubmed
,
Xenbase