Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
p62, a novel Xenopus laevis component of box C/D snoRNPs.
Filippini D
,
Bozzoni I
,
Caffarelli E
.
???displayArticle.abstract???
U16 belongs to the family of box C/D small nucleolar RNAs (snoRNAs) whose members participate in ribosome biogenesis, mainly acting as guides for site-specific methylation of the pre-rRNA. Like all the other members of the family, U16 is associated with a set of protein factors forming a ribonucleoprotein particle, localized in the nucleolus. So far, only a few box C/D-specific proteins are known: in Xenopus laevis, fibrillarin and p68 have been identified by UV crosslinking and shown to require the conserved boxes C and D for snoRNA interaction. In this study, we have identified an additional protein factor (p62), common to box C/D snoRNPs, that crosslinks to the internal stem region, distinct from the conserved box C/D "core motif," of U16 snoRNA. We show here that, although the absence of the core motif and, as a consequence, of fibrillarin and p68 binding prevents processing and accumulation of the snoRNA, the lack of the internal stem does not interfere with the efficient release of U16 from its host intron and only slightly affects snoRNA stability. Because this region is likely to be the binding site for p62, we propose that this protein plays an accessory role in the formation of a mature and stable U16 snoRNP particle.
Bachellerie,
Antisense snoRNAs: a family of nucleolar RNAs with long complementarities to rRNA.
1995, Pubmed
Bachellerie,
Antisense snoRNAs: a family of nucleolar RNAs with long complementarities to rRNA.
1995,
Pubmed
Bachellerie,
Guiding ribose methylation of rRNA.
1997,
Pubmed
Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed
Baserga,
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
1991,
Pubmed
Borovjagin,
U3 small nucleolar RNA is essential for cleavage at sites 1, 2 and 3 in pre-rRNA and determines which rRNA processing pathway is taken in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Caffarelli,
In vitro study of processing of the intron-encoded U16 small nucleolar RNA in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Caffarelli,
Processing of the intron-encoded U16 and U18 snoRNAs: the conserved C and D boxes control both the processing reaction and the stability of the mature snoRNA.
1996,
Pubmed
,
Xenbase
Caffarelli,
In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs.
1998,
Pubmed
,
Xenbase
Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed
Enright,
5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3.
1996,
Pubmed
Fragapane,
A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA.
1993,
Pubmed
,
Xenbase
Ganot,
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
1997,
Pubmed
Gautier,
Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
1997,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Jansen,
Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.
1991,
Pubmed
,
Xenbase
Jarmolowski,
Identification of essential elements in U14 RNA of Saccharomyces cerevisiae.
1990,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Kiss-László,
Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
1998,
Pubmed
Lafontaine,
Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.
1999,
Pubmed
Lange,
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
1998,
Pubmed
,
Xenbase
Maden,
Eukaryotic ribosomal RNA: the recent excitement in the nucleotide modification problem.
1997,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Morrissey,
Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
1993,
Pubmed
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Nicoloso,
U20, a novel small nucleolar RNA, is encoded in an intron of the nucleolin gene in mammals.
1994,
Pubmed
Nicoloso,
Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their direct role as guides for the 2'-O-ribose methylation of rRNAs.
1996,
Pubmed
Peculis,
Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved.
1994,
Pubmed
,
Xenbase
Prislei,
The mechanisms controlling ribosomal protein L1 pre-mRNA splicing are maintained in evolution and rely on conserved intron sequences.
1992,
Pubmed
,
Xenbase
Salim,
Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
1981,
Pubmed
,
Xenbase
Samarsky,
The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization.
1998,
Pubmed
Terns,
A common maturation pathway for small nucleolar RNAs.
1995,
Pubmed
,
Xenbase
Tollervey,
Function and synthesis of small nucleolar RNAs.
1997,
Pubmed
Tollervey,
Small nucleolar RNAs guide ribosomal RNA methylation.
1996,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Tycowski,
A small nucleolar RNA requirement for site-specific ribose methylation of rRNA in Xenopus.
1996,
Pubmed
,
Xenbase
Tycowski,
A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.
1993,
Pubmed
Tycowski,
A mammalian gene with introns instead of exons generating stable RNA products.
1996,
Pubmed
Ware,
Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis.
1983,
Pubmed
,
Xenbase
Watkins,
Elements essential for processing intronic U14 snoRNA are located at the termini of the mature snoRNA sequence and include conserved nucleotide boxes C and D.
1996,
Pubmed
,
Xenbase
Watkins,
In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
1998,
Pubmed
,
Xenbase
Wu,
Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast.
1998,
Pubmed
Xia,
Identification of specific nucleotide sequences and structural elements required for intronic U14 snoRNA processing.
1997,
Pubmed
