Shim S et al. (2000), A novel family of retrotransposons in Xenopus w...

XB-ART-11333

Genesis 2000 Mar 01;263:198-207.

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A novel family of retrotransposons in Xenopus with a developmentally regulated expression.

Shim S , Lee SK , Han JK .

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We have obtained a novel family of LTR-retrotransposons in Xenopus laevis, named Xretpos, from cDNA and genomic clones. Its long terminal repeats (LTRs) can be subdivided into U3, R, and U3 to U5 region, and are bounded by 6 bp inverted repeats. Xretpos contains primer binding site and polypurine tract, and multiple copies of Xretpos-related element are present in the genome. A long open reading frame (ORF) encodes the CCHC motif conserved in retroviral gag proteins and leucine zipper motif capable of forming the coiled-coil. However, no amino acid homology to usually conserved retroviral pol gene was revealed. We report that in vitro synthesized Xretpos complementary RNAs are translated to produce a predicted size of protein. We also show that zygotically activated Xretpos transcripts are restricted to ventro-posterior specific regions and induced by UV-irradiation and BMP-4 overexpression in cycloheximide-dependent way. genesis 26:198-207, 2000.

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Species referenced: Xenopus laevis
Genes referenced: 10a1.1 bmp4

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	FIG. 6. Localization of Xretpos transcripts during early Xenopus development using whole-mount in situ hybridization. (a) Vegetal view of expression at the early gastrula stage (stage 10). Blastopore lip is indicated by an arrow. (b) Dorsal view of an embryo at stage 13. (c) Tailbud stage embryos (stage 25). (d) Tadpole stage (stage 32) embryos. Proctodeum is indicated by an arrow.
	10a1.1 (retrotransposon-like element 10A1 gene 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10, vegetal view, dorsal up.
	10a1.1 (retrotransposon-like element 10A1 gene 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 13, dorsal view, anterior left.
	10a1.1 (retrotransposon-like element 10A1 gene 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 25, lateral view, dorsal up, anterior right.
	10a1.1 (retrotransposon-like element 10A1 gene 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 32, lateral view, dorsal up, anterior right.