XB-ART-11545Cell January 21, 2000; 100 (2): 229-40.
OAZ uses distinct DNA- and protein-binding zinc fingers in separate BMP-Smad and Olf signaling pathways.
We have identified the 30-zinc finger protein OAZ as a DNA-binding factor that associates with Smads in response to BMP2, forming a complex that transcriptionally activates the homeobox regulator of Xenopus mesoderm and neural development, Xvent-2. OAZ contains a BMP signaling module formed by two clusters of fingers that bind Smads and the Xvent-2 BMP response element, respectively. Previously implicated as a transcriptional partner of Olf-1/EBF in olfactory epithelium and lymphocyte development in the rat, OAZ fulfills this role through clusters of fingers that are separate from the BMP signaling module. The mutually exclusive use of OAZ by the BMP-Smad and Olf pathways illustrates the dual role of a multi-zinc finger protein in signal transduction during development.
PubMed ID: 10660046
Article link: Cell
Grant support: CA34610 NCI NIH HHS
Genes referenced: act3 ag1 bmp2 ebf1 ebf2 ebf3 hba1 msx1 nrp1 odc1 smad1 smad4.1 ventx1.1 ventx1.2 ventx2.1 ventx2.2 znf423
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|Figure 2. Subcellular Localization and Tissue Distribution of OAZ (A) OAZ is a nuclear protein. COS-1 cells transfected with Flag-tagged OAZ were analyzed by indirect immunofluorescence using a Flag monoclonal antibody. DAPI staining of DNA is shown for comparison. (B) Temporal expression of OAZ during Xenopus development. RNA isolated from embryos (stages 24) was subjected to RT-PCR using primers to XOAZ, Xvent-2, and ODC (as a loading control). PCR products were visualized by autoradiography. (C) In situ hybridization of XOAZ in Xenopus embryos. At the late neurula stages (a and b), XOAZ expression become restricted to the anterior neural tube. At the tadpole stage (c), XOAZ is expressed at high levels in the forebrain, midbrain, and hindbrain, but not in the spinal cord or the cement gland. (D) Tissue distribution. OAZ mRNA in human tissues.|
|znf423 (zinc finger protein 423) gene expression in Xenopus laevis embryos, NF stage 28, as assayed by in situ hybridization. Lateral view: anterior right, dorsal up.|
|znf423 (zinc finger protein 423) gene expression in Xenopus laevis embryos, NF stage 16, as assayed by in situ hybridization. Anterior view: dorsal up.|