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XB-ART-11592
Development February 1, 2000; 127 (4): 791-800.

Requirement of Sox2-mediated signaling for differentiation of early Xenopus neuroectoderm.

Kishi M , Mizuseki K , Sasai N , Yamazaki H , Shiota K , Nakanishi S , Sasai Y .


Abstract
From early stages of development, Sox2-class transcription factors (Sox1, Sox2 and Sox3) are expressed in neural tissues and sensory epithelia. In this report, we show that Sox2 function is required for neural differentiation of early Xenopus ectoderm. Microinjection of dominant-negative forms of Sox2 (dnSox2) mRNA inhibits neural differentiation of animal caps caused by attenuation of BMP signals. Expression of dnSox2 in developing embryos suppresses expression of N-CAM and regional neural markers. We have analyzed temporal requirement of Sox2-mediated signaling by using an inducible dnSox2 construct fused to the ligand-binding domain of the glucocorticoid receptor. Attenuation of Sox2 function both from the late blastula stage and from the late gastrula stage onwards causes an inhibition of neural differentiation in animal caps and in whole embryos. Additionally, dnSox2-injected cells that fail to differentiate into neural tissues are not able to adopt epidermal cell fate. These data suggest that Sox2-class genes are essential for early neuroectoderm cells to consolidate their neural identity during secondary steps of neural differentiation.

PubMed ID: 10648237
Article link: Development

Genes referenced: actl6a ag1 chrd.1 egr2 en2 fgf4 foxd3 hesx1 hoxb9 krt12.4 myod1 ncam1 neurog2 nr3c1 otx2 snai2 sox1 sox15 sox2 sox3 sox9 tubb2b wi2-2373i1.2 zic2


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