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XB-ART-11594
Nucleic Acids Res February 15, 2000; 28 (4): E12.

A simplified method of generating transgenic Xenopus.



Abstract
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

PubMed ID: 10648800
PMC ID: PMC102591
Article link: Nucleic Acids Res


References:
Christian, 1993, Pubmed, Xenbase [+]


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