XB-ART-11594Nucleic Acids Res February 15, 2000; 28 (4): E12.
A simplified method of generating transgenic Xenopus.
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.
PubMed ID: 10648800
PMC ID: PMC102591
Article link: Nucleic Acids Res