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XB-ART-11615
Dev Biol February 1, 2000; 218 (1): 64-73.

Confocal imaging of early heart development in Xenopus laevis.

Kolker SJ , Tajchman U , Weeks DL .


Abstract
Xenopus laevis provides a number of advantages to studies on cardiovascular development. The embryos are fairly large, are easy to obtain, and can develop at ambient temperature in simple buffer solutions. Although classic descriptions of heart development exist, the ability to use whole-mount immunohistochemical methods and confocal microscopy may enhance the ability to understand both normal and experimentally perturbed cardiovascular development. We have started to examine the early stages of cardiac development in Xenopus, seeking to identify antibodies and fixatives that allow easy examination of the developing heart. We have used monoclonal antibodies (mAbs) raised against bovine cardiac troponin T and chicken tropomyosin to visualize cardiac muscle, a goat antibody recognizing bovine type VI collagen to stain the lining of vessels, and the JB3 mAb raised against chicken fibrillin, which allows the visualization of a variety of cardiovascular tissues during early development. Results from embryonic stages 24-46 are presented.

PubMed ID: 10644411
PMC ID: PMC3568754
Article link: Dev Biol
Grant support: HL42252 NHLBI NIH HHS , P50 HL062178-050003 NHLBI NIH HHS , P50 HL062178 NHLBI NIH HHS

Genes referenced: col6a1 fbn1 fbn2 fn1 tnnt2 tpm1
Antibodies referenced: Col6a1 Ab1 Fbn2 Ab1 Tnnt2 Ab1 Tpm1 Ab1



Article Images: [+] show captions
References:
Cleaver, 1997, Pubmed, Xenbase [+]


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