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XB-ART-11709
Nat Cell Biol January 1, 2000; 2 (1): 13-9.

Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.

Tournebize R , Popov A , Kinoshita K , Ashford AJ , Rybina S , Pozniakovsky A , Mayer TU , Walczak CE , Karsenti E , Hyman AA .


Abstract
Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

PubMed ID: 10620801
Article link: Nat Cell Biol
Grant support: [+]
Genes referenced: ckap5 kif2c

References :
Heald, A dynamic duo of microtubule modulators. 2000, Pubmed, Xenbase


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