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We have examined the distribution of RNA transcription and processing factors in the amphibian oocytenucleus or germinal vesicle. RNA polymerase I (pol I), pol II, and pol III occur in the Cajal bodies (coiled bodies) along with various components required for transcription and processing of the three classes of nuclear transcripts: mRNA, rRNA, and pol III transcripts. Among these components are transcription factor IIF (TFIIF), TFIIS, splicing factors, the U7 small nuclear ribonucleoprotein particle, the stem-loop binding protein, SR proteins, cleavage and polyadenylation factors, small nucleolar RNAs, nucleolar proteins that are probably involved in pre-rRNA processing, and TFIIIA. Earlier studies and data presented here show that several of these components are first targeted to Cajal bodies when injected into the oocyte and only subsequently appear in the chromosomes or nucleoli, where transcription itself occurs. We suggest that pol I, pol II, and pol III transcription and processing components are preassembled in Cajal bodies before transport to the chromosomes and nucleoli. Most components of the pol II transcription and processing pathway that occur in Cajal bodies are also found in the many hundreds of B-snurposomes in the germinal vesicle. Electron microscopic images show that B-snurposomes consist primarily, if not exclusively, of 20- to 30-nm particles, which closely resemble the interchromatin granules described from sections of somatic nuclei. We suggest the name pol II transcriptosome for these particles to emphasize their content of factors involved in synthesis and processing of mRNA transcripts. We present a model in which pol I, pol II, and pol III transcriptosomes are assembled in the Cajal bodies before export to the nucleolus (pol I), to the B-snurposomes and eventually to the chromosomes (pol II), and directly to the chromosomes (pol III). The key feature of this model is the preassembly of the transcription and processing machinery into unitary particles. An analogy can be made between ribosomes and transcriptosomes, ribosomes being unitary particles involved in translation and transcriptosomes being unitary particles for transcription and processing of RNA.
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999, Pubmed,
Xenbase
Abbott,
The stem-loop binding protein (SLBP1) is present in coiled bodies of the Xenopus germinal vesicle.
1999,
Pubmed
,
Xenbase
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991,
Pubmed
Bachellerie,
Guiding ribose methylation of rRNA.
1997,
Pubmed
Bauer,
In vitro assembly of coiled bodies in Xenopus egg extract.
1994,
Pubmed
,
Xenbase
Bellini,
Coilin can form a complex with the U7 small nuclear ribonucleoprotein.
1998,
Pubmed
,
Xenbase
Bentley,
Coupling RNA polymerase II transcription with pre-mRNA processing.
1999,
Pubmed
Bier,
[Structure and function of oocyte chromosomes nucleoli and as well as the extra DNA during oogenesis in panoistic and meroistic insects].
1967,
Pubmed
Bohmann,
Molecular analysis of the coiled body.
1995,
Pubmed
Bregman,
Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains.
1995,
Pubmed
Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed
Bucci,
Incorporation of tritiated uridine in nuclei of Triturus oocytes treated with alpha-amanitin.
1971,
Pubmed
Callan,
Lampbrush chromosomes.
1986,
Pubmed
Callan,
The lampbrush chromosomes of Xenopus laevis: preparation, identification, and distribution of 5S DNA sequences.
1987,
Pubmed
,
Xenbase
Callan,
Association of RNA with the B and C snurposomes of Xenopus oocyte nuclei.
1991,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Fakan,
The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and distribution.
1980,
Pubmed
Frey,
Coiled bodies contain U7 small nuclear RNA and associate with specific DNA sequences in interphase human cells.
1995,
Pubmed
Fu,
Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
1990,
Pubmed
GALL,
H3 uridine incorporation in lampbrush chromosomes.
1962,
Pubmed
Gall,
Differential synthesis of the genes for ribosomal RNA during amphibian oögenesis.
1968,
Pubmed
Gall,
Is the sphere organelle/coiled body a universal nuclear component?
1995,
Pubmed
,
Xenbase
Grande,
Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II.
1997,
Pubmed
Greenblatt,
RNA polymerase II holoenzyme and transcriptional regulation.
1997,
Pubmed
Greenleaf,
Positive patches and negative noodles: linking RNA processing to transcription?
1993,
Pubmed
Halle,
Gene expression: increasing evidence for a transcriptosome.
1996,
Pubmed
Hannan,
Affinity purification of mammalian RNA polymerase I. Identification of an associated kinase.
1998,
Pubmed
IZAWA,
The relationship between RNA synthesis and loop structure in lampbrush chromosomes.
1963,
Pubmed
Jackson,
Visualization of focal sites of transcription within human nuclei.
1993,
Pubmed
Jantsch,
Assembly and localization of the U1-specific snRNP C protein in the amphibian oocyte.
1992,
Pubmed
,
Xenbase
Jenny,
Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit.
1994,
Pubmed
Jiménez-García,
Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis.
1994,
Pubmed
Jordan,
In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive.
1996,
Pubmed
Kazmaier,
Functional characterization of X. laevis U5 snRNA genes.
1987,
Pubmed
,
Xenbase
Kim,
Splicing factors associate with hyperphosphorylated RNA polymerase II in the absence of pre-mRNA.
1997,
Pubmed
Lacroix,
Monoclonal antibodies to lampbrush chromosome antigens of Pleurodeles waltlii.
1985,
Pubmed
Lamond,
Structure and function in the nucleus.
1998,
Pubmed
Lange,
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
1998,
Pubmed
,
Xenbase
Lange,
Nucleolar localization elements in U8 snoRNA differ from sequences required for rRNA processing.
1998,
Pubmed
,
Xenbase
Lange,
Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.
1998,
Pubmed
,
Xenbase
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Macgregor,
Pattern of incorporation of (3H)uridine into RNA of amphibian oocyte nucleoli.
1967,
Pubmed
Macgregor,
The nucleolus and its genes in amphibian oogenesis.
1972,
Pubmed
,
Xenbase
Matera,
Of coiled bodies, gems, and salmon.
1998,
Pubmed
Matera,
Organization of small nucleolar ribonucleoproteins (snoRNPs) by fluorescence in situ hybridization and immunocytochemistry.
1994,
Pubmed
Matera,
Nuclear bodies: multifaceted subdomains of the interchromatin space.
1999,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Meier,
Nopp140 shuttles on tracks between nucleolus and cytoplasm.
1992,
Pubmed
Meier,
NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria.
1994,
Pubmed
Messmer,
Requirements for nuclear translocation and nucleolar accumulation of nucleolin of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Miller,
Structure and composition of peripheral nucleoli of salamander oocytes.
1966,
Pubmed
Miller,
Visualization of RNA synthesis on chromosomes.
1972,
Pubmed
Mintz,
Purification and biochemical characterization of interchromatin granule clusters.
1999,
Pubmed
Minvielle-Sebastia,
mRNA polyadenylation and its coupling to other RNA processing reactions and to transcription.
1999,
Pubmed
Misteli,
The cellular organization of gene expression.
1998,
Pubmed
Monneron,
Fine structural organization of the interphase nucleus in some mammalian cells.
1969,
Pubmed
Myer,
RNA polymerase II holoenzymes and subcomplexes.
1998,
Pubmed
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Parvin,
Regulatory targets in the RNA polymerase II holoenzyme.
1998,
Pubmed
Patturajan,
Growth-related changes in phosphorylation of yeast RNA polymerase II.
1998,
Pubmed
Peculis,
Localization of the nucleolar protein NO38 in amphibian oocytes.
1992,
Pubmed
,
Xenbase
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Pollard,
The autoimmunity-inducing xenobiotic mercury interacts with the autoantigen fibrillarin and modifies its molecular and antigenic properties.
1997,
Pubmed
Pombo,
Regional specialization in human nuclei: visualization of discrete sites of transcription by RNA polymerase III.
1999,
Pubmed
Raska,
Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies.
1991,
Pubmed
Roeder,
Role of general and gene-specific cofactors in the regulation of eukaryotic transcription.
1998,
Pubmed
Roth,
Monoclonal antibodies that recognize transcription unit proteins on newt lampbrush chromosomes.
1987,
Pubmed
Roth,
Targeting of a chromosomal protein to the nucleus and to lampbrush chromosome loops.
1989,
Pubmed
,
Xenbase
Roth,
A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle.
1990,
Pubmed
,
Xenbase
Roth,
A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription.
1991,
Pubmed
,
Xenbase
Samarsky,
The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization.
1998,
Pubmed
Scheer,
Structure and function of the nucleolus.
1999,
Pubmed
Schmidt-Zachmann,
Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.
1984,
Pubmed
,
Xenbase
Schmidt-Zachmann,
A constitutive nucleolar protein identified as a member of the nucleoplasmin family.
1987,
Pubmed
,
Xenbase
Schul,
Nuclear domains enriched in RNA 3'-processing factors associate with coiled bodies and histone genes in a cell cycle-dependent manner.
1999,
Pubmed
Schul,
Coiled bodies and U2 snRNA genes adjacent to coiled bodies are enriched in factors required for snRNA transcription.
1998,
Pubmed
Schul,
The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA.
1996,
Pubmed
Schultz,
In vitro RNA synthesis in oocyte nuclei of the newt Notophthalmus.
1981,
Pubmed
Seither,
Mammalian RNA polymerase I exists as a holoenzyme with associated basal transcription factors.
1998,
Pubmed
Shaw,
Localization and processing from a polycistronic precursor of novel snoRNAs in maize.
1998,
Pubmed
Smith,
Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.
1991,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Spector,
Macromolecular domains within the cell nucleus.
1993,
Pubmed
Steinmetz,
Pre-mRNA processing and the CTD of RNA polymerase II: the tail that wags the dog?
1997,
Pubmed
Takagaki,
A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein.
1994,
Pubmed
Thompson,
Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II.
1989,
Pubmed
,
Xenbase
Tsvetkov,
Transcription on lampbrush chromosome loops in the absence of U2 snRNA.
1992,
Pubmed
,
Xenbase
Tuma,
Identification and characterization of a sphere organelle protein.
1993,
Pubmed
,
Xenbase
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wang,
Identification of an autonomously initiating RNA polymerase III holoenzyme containing a novel factor that is selectively inactivated during protein synthesis inhibition.
1997,
Pubmed
Wansink,
Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus.
1993,
Pubmed
Weinstein,
Guided tours: from precursor snoRNA to functional snoRNP.
1999,
Pubmed
Wu,
U7 small nuclear RNA in C snurposomes of the Xenopus germinal vesicle.
1993,
Pubmed
,
Xenbase
Wu,
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
1991,
Pubmed
,
Xenbase
Wu,
Snurposomes and coiled bodies.
1993,
Pubmed
,
Xenbase
Wu,
The Sm binding site targets U7 snRNA to coiled bodies (spheres) of amphibian oocytes.
1996,
Pubmed
,
Xenbase
Zahler,
SR proteins: a conserved family of pre-mRNA splicing factors.
1992,
Pubmed
de Jong,
Nuclear domains involved in RNA synthesis, RNA processing, and replication.
1996,
Pubmed
