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XB-ART-11997
Science. November 12, 1999; 286 (5443): The protein kinase p90 rsk as an essential mediator of cytostatic factor activity.
Bhatt RR
,
Ferrell JE
.
Abstract Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos-MEK-1-p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.Pubmed Id: 10558991 Article link: Science.Grant support:
GM16415 NIGMS NIH HHS , GM46383 NIGMS NIH HHS Genes referenced: cdk20 mapk1 mos rps6ka1 rps6ka3 Antibodies referenced: |
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