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Two paralogous mitochondrial malate dehydrogenase 2 (Mdh2) genes of Xenopus laevis have been cloned and sequenced, revealing 95% identity. Fluorescence in-situ hybridization (FISH) combined with tyramide amplification discriminates both genes; Mdh2a was localized into chromosome q3 and Mdh2b into chromosome q8. One kb cDNA probes detect both genes with 85% accuracy. The remaining signals were on the paralogous counterpart. Introns interrupt coding sequences at the same nucleotide as defined for mouse. Restriction polymorphism has been detected in the first intron of Mdh2a, while the individual variability in intron 6 of Mdh2b gene is represented by an insertion of incomplete retrotransposon L1Xl. Rates of nucleotide substitutions indicate that both genes are under similar evolutionary constraints. X. laevis Mdh2 genes can be used as markers for physical mapping and linkage analysis.
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977, Pubmed,
Xenbase
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Castresana,
Estimation of genetic distances from human and mouse introns.
2002,
Pubmed
Courtet,
Major histocompatibility complex and immunoglobulin loci visualized by in situ hybridization on Xenopus chromosomes.
2001,
Pubmed
,
Xenbase
Emanuelsson,
Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
2000,
Pubmed
Evans,
A mitochondrial DNA phylogeny of African clawed frogs: phylogeography and implications for polyploid evolution.
2004,
Pubmed
,
Xenbase
Force,
Preservation of duplicate genes by complementary, degenerative mutations.
1999,
Pubmed
Fujiwara,
Uniparental chromosome elimination in the early embryogenesis of the inviable salmonid hybrids between masu salmon female and rainbow trout male.
1997,
Pubmed
Goward,
Malate dehydrogenase: a model for structure, evolution, and catalysis.
1994,
Pubmed
Graf,
Genetics of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Hikosaka,
Lineage-specific tandem repeats riding on a transposable element of MITE in Xenopus evolution: a new mechanism for creating simple sequence repeats.
2004,
Pubmed
,
Xenbase
Hughes,
Evolution of duplicate genes in a tetraploid animal, Xenopus laevis.
1993,
Pubmed
,
Xenbase
Krylov,
The c-SRC1 gene visualized by in situ hybridization on Xenopus laevis chromosomes.
2003,
Pubmed
,
Xenbase
Kubota,
Germ line-restricted, highly repeated DNA sequences and their chromosomal localization in a Japanese hagfish (Eptatretus okinoseanus).
1993,
Pubmed
Kumar,
MEGA2: molecular evolutionary genetics analysis software.
2001,
Pubmed
Matsuda,
Identification of the sex chromosomes of the medaka, Oryzias latipes, by fluorescence in situ hybridization.
1998,
Pubmed
McAlister-Henn,
Complete nucleotide sequence of the Escherichia coli gene encoding malate dehydrogenase.
1987,
Pubmed
Mácha,
Xstir polymorphism and absence of sex linkage in Xenopus laevis ME2 gene.
2003,
Pubmed
,
Xenbase
Müller,
The lampbrush chromosomes of Xenopus laevis (Daudin).
1974,
Pubmed
,
Xenbase
Müller,
Conservation of the positions of metazoan introns from sponges to humans.
2002,
Pubmed
Nanda,
Amplification of a long terminal repeat-like element on the Y chromosome of the platyfish, Xiphophorus maculatus.
2000,
Pubmed
Nanda,
Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences.
1992,
Pubmed
Nei,
Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions.
1986,
Pubmed
Odierna,
Evidence for chromosome and Pst I satellite DNA family evolutionary stasis in the Bufo viridis group (Amphibia, Anura).
2004,
Pubmed
Owens,
Cloning of the 18S rDNA gene, an internal transcribed spacer, and the 5' region of the 28S rDNA gene of Cope's gray treefrog, Hyla chrysoscelis.
2001,
Pubmed
Phillips,
Physical and genetic mapping of the rainbow trout major histocompatibility regions: evidence for duplication of the class I region.
2003,
Pubmed
Phillips,
Localization of repetitive DNAs to zebrafish (Danio rerio) chromosomes by fluorescence in situ hybridization (FISH).
2000,
Pubmed
Picariello,
S1 satellite DNA as a taxonomic marker in brown frogs: molecular evidence that Rana graeca graeca and Rana graeca italica are different species.
2002,
Pubmed
Reed,
Molecular characterization and cytogenetic analysis of highly repeated DNAs of lake trout, Salvelinus namaycush.
1995,
Pubmed
Roest Crollius,
Characterization and repeat analysis of the compact genome of the freshwater pufferfish Tetraodon nigroviridis.
2000,
Pubmed
Schmid,
Chromosome banding in Amphibia. XVI. High-resolution replication banding patterns in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Setoyama,
Structural organization of the mouse cytosolic malate dehydrogenase gene: comparison with that of the mouse mitochondrial malate dehydrogenase gene.
1988,
Pubmed
Sherman,
Human immunodeficiency virus integration protein expressed in Escherichia coli possesses selective DNA cleaving activity.
1990,
Pubmed
Stein,
Identification of the Y chromosome in chinook salmon (Oncorhynchus tshawytscha).
2001,
Pubmed
Tymowska,
Chromosome complements of the genus Xenopus.
1973,
Pubmed
,
Xenbase
Tymowska,
Karyotype analysis of Xenopus muelleri (Peters) and Xenopus laevis (Daudin), Pipidae.
1972,
Pubmed
,
Xenbase
Wiley,
Replication banding and FISH analysis reveal the origin of the Hyla femoralis karyotype and XY/XX sex chromosomes.
2003,
Pubmed
