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XB-ART-12312
Biol Reprod 1999 Oct 01;614:912-20.
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Efficient human sperm pronucleus formation and replication in Xenopus egg extracts.

Neuber E , Havari E , Aquiles Sanchez J , Powers RD , Wangh LJ .


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We have achieved efficient in vitro reactivation and replication of human sperm nuclei in frog egg extracts by constructing a 4-step protocol that mimics the events of fertilization and pronucleus formation in mammalian eggs. With use of this protocol, 78-97% of human sperm nuclei from fertile donors synchronously swelled and completed full genome replication in about 2 h. We document the changes in nuclear structure that accompany efficient DNA synthesis and discuss future research and potential clinical implications of this new system.

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