XB-ART-12515Mech Dev August 1, 1999; 86 (1-2): 203-7.
A family of genes related to the Drosophila wingless receptor frizzled have been found in vertebrates. We have cloned full length cDNAs of two novel frizzled genes from embryonic Xenopus tissue. We are calling them Xfz7 and Xfz9 (for Xenopus frizzled) because their deduced peptide sequences show extensive similarity to other vertebrate frizzled molecules. Xfz7 is closely related to human, chick and mouse frz-7 and Xfz9 is most related to human FZD9 and mouse fzd9. Xfz7 is expressed in a broad, complex and dynamic pattern beginning at gastrulation. At later stages Xfz7 expression is found in neural crest, neural tube, eye, pronephric duct and the heart. Xfz9 expression in contrast is more restricted to the neuroectoderm and, at later stages of development, to the dorsal regions of the mid- and hindbrain.
PubMed ID: 10446283
Article link: Mech Dev
Genes referenced: fzd10 fzd7 fzd9
Article Images: [+] show captions
|Fig. 2. Whole mount in situ hybridisation using Xfz7 antisense RNA probe. (A) stage 11, (B) stage 18, lateral view, anterior to left, dorsal to top (C) stage 18, head on anterior view, (D) stage 25, (E) stage 29, (F) stage 29 higher magnification view of head region, (G) stage 33/34, (H) stage 35, higher magnification view of pronephric duct and somitic region of trunk, anterior to left, dorsal to top. (I–P) 20 μm transverse sections, dorsal to top. (I stage 11.5, (J) stage 18, (K–M) stage 29, (K) section through anterior head region, (L) more posterior section through head region (M) section through trunk region, (N) stage 31, (O) stage 30 close up view of heart, (P) stage 33/34 close up view of heart. ba, branchial arches; bl, blastopore lip; D, dorsal; e, eye; en, endocardium; end, endoderm; h, heart; hb, hindbrain; hf, heart field; hnc, hyoid neural crest; isa, intersomitic arteries; im, lateral mesoderm; m, myocardium; mes, mesoderm; mnc, mandibular neural crest; nc, neural crest; ne, neuroectoderm; not, notochord; nt, neural tube; p, pericardium; pn, pronephros; pnd, pronephric duct; sm, somitic mesoderm; tb, tailbud; V, ventral.|
|Fig. 3. Whole mount in situ hybridisation using Xfz9 antisense RNA probe. (A) stage 18, dorsal view with anterior to top, (B) stage 22, top dorsal view, bottom lateral view, anterior to left (C) stage 26, dorsal view, (D) stage 35/36. (E–G) 20 μm transverse sections, anterior to top. (E) stage 16, (F,G) stage 33/34, (F) head region, (G) more posterior head region. dnt, dorsal neural tube; hb, hindbrain; mb, midbrain; ne, neuroectoderm; tb, tailbud.|