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Cell July 23, 1999; 98 (2): 239-48.

13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation.

Kimura K , Rybenkov VV , Crisona NJ , Hirano T , Cozzarelli NR .

Xenopus 13S condensin converts interphase chromatin into mitotic-like chromosomes, and, in the presence of ATP and a type I topoisomerase, introduces (+) supercoils into DNA. The specific production of (+) trefoil knots in the presence of condensin and a type II topoisomerase shows that condensin reconfigures DNA by introducing an ordered, global, (+) writhe. Knotting required ATP hydrolysis and cell cycle-specific phosphorylation of condensin. Condensin bound preferentially to (+) supercoiled DNA in the presence of ATP but not in its absence. Our results suggest a mechanism for the compaction of chromatin by condensin during mitosis.

PubMed ID: 10428035
Article link: Cell
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