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Nuclear export in plants. Use of geminivirus movement proteins for a cell-based export assay.
Ward BM
,
Lazarowitz SG
.
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The nuclear export of proteins and RNAs has been studied in heterokaryons or by microinjecting test substrates into nuclei of HeLa cells or Xenopus oocytes. We have previously shown that the two movement proteins BR1 and BL1 encoded by the plant pathogenic squash leaf curl virus act in a coordinated manner to facilitate virus cell-to-cell movement and that one of these (BR1) is a nuclear shuttle protein. By using a novel in vivo cell-based assay for nuclear export in which nuclear-localized BR1 is trapped by BL1 and redirected to the cortical cytoplasm, we demonstrate that residues 177 to 198 of BR1 contain a leucine-rich nuclear export signal (NES) of the type found in the Rev protein encoded by the human immunodeficiency virus and in Xenopus TFIIIA. We further show that the TFIIIA NES can functionally replace the NES of BR1 in both nuclear export and viral infectivity. These findings suggest that this basic pathway for nuclear export is highly conserved among plant and animal cells and in yeast.
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990, Pubmed,
Xenbase
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990,
Pubmed
,
Xenbase
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase
Corbett,
Genetic analysis of macromolecular transport across the nuclear envelope.
1996,
Pubmed
Corbett,
Nucleocytoplasmic transport of macromolecules.
1997,
Pubmed
Davis,
The nuclear pore complex.
1995,
Pubmed
,
Xenbase
Deane,
Ran-binding protein 5 (RanBP5) is related to the nuclear transport factor importin-beta but interacts differently with RanBP1.
1997,
Pubmed
Dingwall,
In vitro systems for the reconstitution of snRNP and protein nuclear import.
1998,
Pubmed
Dobbelstein,
Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence.
1997,
Pubmed
Featherstone,
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
1988,
Pubmed
,
Xenbase
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Forbes,
Structure and function of the nuclear pore complex.
1992,
Pubmed
,
Xenbase
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fridell,
Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev.
1996,
Pubmed
,
Xenbase
Fridell,
Nuclear import of hnRNP A1 is mediated by a novel cellular cofactor related to karyopherin-beta.
1997,
Pubmed
Fridell,
Nuclear export of late HIV-1 mRNAs occurs via a cellular protein export pathway.
1996,
Pubmed
GOLDSTEIN,
Localization of nucleusspecific protein as shown by transplantation experiments in Amoeba proteus.
1958,
Pubmed
Gerace,
Nuclear export signals and the fast track to the cytoplasm.
1995,
Pubmed
Görlich,
Nuclear protein import.
1997,
Pubmed
Görlich,
Importin provides a link between nuclear protein import and U snRNA export.
1996,
Pubmed
,
Xenbase
Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed
Haizel,
Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis.
1997,
Pubmed
Hicks,
Protein import into the nucleus: an integrated view.
1995,
Pubmed
Hicks,
Nuclear import in permeabilized protoplasts from higher plants has unique features.
1996,
Pubmed
Hicks,
Three classes of nuclear import signals bind to plant nuclei.
1995,
Pubmed
Ingham,
Both bipartite geminivirus movement proteins define viral host range, but only BL1 determines viral pathogenicity.
1995,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Kehlenbach,
Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro.
1998,
Pubmed
Lazarowitz,
Infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite squash leaf curl geminivirus with a broad host range phenotype.
1991,
Pubmed
Lewis,
A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.
1996,
Pubmed
Liang,
Adenoviral E1B-55kDa protein inhibits yeast mRNA export and perturbs nuclear structure.
1995,
Pubmed
Martin,
Nuclear transport of influenza virus ribonucleoproteins: the viral matrix protein (M1) promotes export and inhibits import.
1991,
Pubmed
McGarvey,
A simple and rapid method for screening transgenic plants using the PCR.
1991,
Pubmed
Melchior,
Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor.
1993,
Pubmed
Merkle,
A plant in vitro system for the nuclear import of proteins.
1996,
Pubmed
Michael,
The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hnRNP K protein.
1997,
Pubmed
,
Xenbase
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Nakielny,
Transportin: nuclear transport receptor of a novel nuclear protein import pathway.
1996,
Pubmed
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Noueiry,
Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport.
1994,
Pubmed
Pascal,
Transgenic tobacco plants expressing the geminivirus BL1 protein exhibit symptoms of viral disease.
1993,
Pubmed
Pascal,
The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus.
1994,
Pubmed
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Restrepo,
Nuclear transport of plant potyviral proteins.
1990,
Pubmed
Sanderfoot,
Cooperation in Viral Movement: The Geminivirus BL1 Movement Protein Interacts with BR1 and Redirects It from the Nucleus to the Cell Periphery.
1995,
Pubmed
Sanderfoot,
Getting it together in plant virus movement: cooperative interactions between bipartite geminivirus movement proteins.
1996,
Pubmed
Sanderfoot,
A viral movement protein as a nuclear shuttle. The geminivirus BR1 movement protein contains domains essential for interaction with BL1 and nuclear localization.
1996,
Pubmed
Sandri-Goldin,
ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.
1998,
Pubmed
Schlenstedt,
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
1997,
Pubmed
Senger,
Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p.
1998,
Pubmed
Snay-Hodge,
Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export.
1998,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Stutz,
Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast.
1995,
Pubmed
Stutz,
A functional interaction between Rev and yeast pre-mRNA is related to splicing complex formation.
1994,
Pubmed
Truant,
Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein.
1998,
Pubmed
Tseng,
Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export.
1998,
Pubmed
,
Xenbase
Varagona,
Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2.
1992,
Pubmed
Vrana,
Mapping functional regions of transcription factor TFIIIA.
1988,
Pubmed
,
Xenbase
Ward,
The geminivirus BL1 movement protein is associated with endoplasmic reticulum-derived tubules in developing phloem cells.
1997,
Pubmed
Weis,
Importins and exportins: how to get in and out of the nucleus.
1998,
Pubmed
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Whittaker,
Nuclear trafficking of influenza virus ribonuleoproteins in heterokaryons.
1996,
Pubmed
Yang,
Subnuclear trafficking of glucocorticoid receptors in vitro: chromatin recycling and nuclear export.
1997,
Pubmed
