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Plant Mol Biol
1999 May 01;401:99-110. doi: 10.1023/a:1026405311132.
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Characterization of the pea rDNA replication fork barrier: putative cis-acting and trans-acting factors.
López-Estraño C
,
Schvartzman JB
,
Krimer DB
,
Hernández P
.
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It was previously shown that in pea (Pisum sativum), rDNA repeats contain a polar replication fork barrier that blocks progression of the replication machinery moving in the direction opposite to transcription. This barrier maps in the untranscribed spacer close to the 3' end of the 25S gene. Very similar barriers are also found in the rDNA of yeast, Xenopus and mammalian cultured cells. This high conservation indicates that the rDNA barrier plays a relevant biological role. Progression of replication forks through the DNA sequence where the barrier maps in pea was investigated in plasmids replicating in Escherichia coli and Saccharomyces cerevisiae. No barrier was detected in these heterologous systems, indicating that the DNA sequence by itself was insufficient to block the replication machinery. Therefore, trans-acting factors were likely to be required. Taking advantage of the natural sequence heterogeneity in pea rDNA, we obtained evidence that a 27 bp imperfect tandem repeat is involved in the arrest of replication. Moreover, nuclear protein(s) specifically bound to this repeat suggesting that this DNA/protein complex is responsible for the polar arrest of replication forks.
Bierne,
When replication forks stop.
1994,
Pubmed
Brewer,
A replication fork barrier at the 3' end of yeast ribosomal RNA genes.
1988,
Pubmed
Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed
Brewer,
The arrest of replication forks in the rDNA of yeast occurs independently of transcription.
1992,
Pubmed
Dayn,
Intramolecular DNA triplexes: unusual sequence requirements and influence on DNA polymerization.
1992,
Pubmed
Fangman,
Activation of replication origins within yeast chromosomes.
1991,
Pubmed
Gerber,
Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I.
1997,
Pubmed
Hernández,
Replication termini in the rDNA of synchronized pea root cells (Pisum sativum).
1988,
Pubmed
Hernández,
Proximity of an ARS consensus sequence to a replication origin of pea (Pisum sativum).
1988,
Pubmed
Hernández,
Conserved features in the mode of replication of eukaryotic ribosomal RNA genes.
1993,
Pubmed
Huberman,
The in vivo replication origin of the yeast 2 microns plasmid.
1987,
Pubmed
Ingle,
The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants.
1975,
Pubmed
Jorgensen,
Structure and variation in ribosomal RNA genes of pea : Characterization of a cloned rDNA repeat and chromosomal rDNA variants.
1987,
Pubmed
Linskens,
Organization of replication of ribosomal DNA in Saccharomyces cerevisiae.
1988,
Pubmed
Little,
Initiation and termination of DNA replication in human rRNA genes.
1993,
Pubmed
López-estraño,
Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA.
1998,
Pubmed
Piller,
Structural analysis of the short length ribosomal DNA variant from Pisum sativum L. cv. Alaska.
1990,
Pubmed
Polans,
Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea.
1986,
Pubmed
Rao,
Pausing of simian virus 40 DNA replication fork movement in vivo by (dG-dA)n.(dT-dC)n tracts.
1994,
Pubmed
Samadashwily,
Trinucleotide repeats affect DNA replication in vivo.
1997,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schiestl,
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
1989,
Pubmed
Viguera,
The ColE1 unidirectional origin acts as a polar replication fork pausing site.
1996,
Pubmed
Weaver,
The role of palindromic and non-palindromic sequences in arresting DNA synthesis in vitro and in vivo.
1984,
Pubmed
Wiesendanger,
Replication fork barriers in the Xenopus rDNA.
1994,
Pubmed
,
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