XB-ART-12943J Biochem June 1, 1999; 125 (6): 1175-82.
Xenopus maintenance-type DNA methyltransferase is accumulated and translocated into germinal vesicles of oocytes.
In vertebrates, DNA methylation plays an important role in the regulation of gene expression and embryogenesis. DNA methyltransferase, which catalyzes the introduction of a methyl group at the 5th position of cytosine in the CpG sequence, is highly accumulated in mouse oocytes and is excluded from nuclei [Carlson et al. (1992) Genes Dev. 6, 2536-2541]. In this study, we examined the expression level and localization of Xenopus DNA methyltransferase in oocytes during oogenesis. The DNA methyltransferase protein was detectable in stage III oocytes and increased thereafter, until the oocytes had matured. The rate of DNA methyltransferase synthesis rapidly increased after stage IV oocytes. Different from in mouse oocytes, DNA methyltransferase was equally distributed in the nuclear and post-nuclear fractions, in stage VI oocytes. DNA methyltransferase translocated into nuclei was uniformly localized in the nuclear matrix, and the accumulated DNA methyltransferase in stage VI nuclei had DNA methylation activity.
PubMed ID: 10348922
Article link: J Biochem
Genes referenced: tbx2