XB-ART-12993Science May 21, 1999; 284 (5418): 1356-8.
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
PubMed ID: 10334990
Article link: Science
Genes referenced: ran rcc1