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Proc Natl Acad Sci U S A
1999 Apr 13;968:4718-23. doi: 10.1073/pnas.96.8.4718.
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Genes for calcineurin B-like proteins in Arabidopsis are differentially regulated by stress signals.
Kudla J
,
Xu Q
,
Harter K
,
Gruissem W
,
Luan S
.
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An important effector of Ca2+ signaling in animals and yeast is the Ca2+/calmodulin-dependent protein phosphatase calcineurin. However, the biochemical identity of plant calcineurin remained elusive. Here we report the molecular characterization of AtCBL (Arabidopsis thaliana calcineurin B-like protein) from Arabidopsis. The protein is most similar to mammalian calcineurin B, the regulatory subunit of the phosphatase. AtCBL also shows significant similarity with another Ca2+-binding protein, the neuronal calcium sensor in animals. It contains typical EF-hand motifs with Ca2+-binding capability, as confirmed by in vitro Ca2+-binding assays, and it interacts in vivo with rat calcineurin A in the yeast two-hybrid system. Interaction of AtCBL1 and rat calcineurin A complemented the salt-sensitive phenotype in a yeast calcineurin B mutant. Cloning of cDNAs revealed that AtCBL proteins are encoded by a family of at least six genes in Arabidopsis. Genes for three isoforms were identified in this study. AtCBL1 mRNA was preferentially expressed in stems and roots and its mRNA levels strongly increased in response to specific stress signals such as drought, cold, and wounding. In contrast, AtCBL2 and AtCBL3 are constitutively expressed under all conditions investigated. Our data suggest that AtCBL1 may act as a regulatory subunit of a plant calcineurin-like activity mediating calcium signaling under certain stress conditions.
Allen,
Calcineurin, a Type 2B Protein Phosphatase, Modulates the Ca2+-Permeable Slow Vacuolar Ion Channel of Stomatal Guard Cells.
1995, Pubmed
Allen,
Calcineurin, a Type 2B Protein Phosphatase, Modulates the Ca2+-Permeable Slow Vacuolar Ion Channel of Stomatal Guard Cells.
1995,
Pubmed
Breeden,
Regulation of the yeast HO gene.
1985,
Pubmed
Cardenas,
Targets of immunophilin-immunosuppressant complexes are distinct highly conserved regions of calcineurin A.
1995,
Pubmed
Cardenas,
Immunophilins interact with calcineurin in the absence of exogenous immunosuppressive ligands.
1994,
Pubmed
Chang,
Asymmetric retraction of growth cone filopodia following focal inactivation of calcineurin.
1995,
Pubmed
Clapham,
Calcium signaling.
1995,
Pubmed
Elledge,
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
1991,
Pubmed
Foor,
Calcineurin mediates inhibition by FK506 and cyclosporin of recovery from alpha-factor arrest in yeast.
1992,
Pubmed
Gietz,
Improved method for high efficiency transformation of intact yeast cells.
1992,
Pubmed
Griffith,
X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex.
1995,
Pubmed
Guarente,
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
1983,
Pubmed
Guerini,
Calcineurin: not just a simple protein phosphatase.
1997,
Pubmed
Hunter,
Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.
1995,
Pubmed
Iwabuchi,
Use of the two-hybrid system to identify the domain of p53 involved in oligomerization.
1993,
Pubmed
Kieber,
CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.
1993,
Pubmed
Kissinger,
Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex.
1995,
Pubmed
Klee,
Calcineurin.
1988,
Pubmed
Knight,
Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation.
1996,
Pubmed
Knight,
Calcium signalling in Arabidopsis thaliana responding to drought and salinity.
1997,
Pubmed
Krinks,
Identification and quantification of calcium-binding proteins in squid axoplasm.
1988,
Pubmed
Liu,
A calcium sensor homolog required for plant salt tolerance.
1998,
Pubmed
Liu,
Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes.
1991,
Pubmed
Luan,
pCyP B: a chloroplast-localized, heat shock-responsive cyclophilin from fava bean.
1994,
Pubmed
Luan,
Immunosuppressants implicate protein phosphatase regulation of K+ channels in guard cells.
1993,
Pubmed
Luan,
Molecular characterization of a FKBP-type immunophilin from higher plants.
1996,
Pubmed
Nakamura,
Protein phosphatase type 2B (calcineurin)-mediated, FK506-sensitive regulation of intracellular ions in yeast is an important determinant for adaptation to high salt stress conditions.
1993,
Pubmed
Olafsson,
Molecular cloning and functional characterization of the Xenopus Ca(2+)-binding protein frequenin.
1995,
Pubmed
,
Xenbase
Pardo,
Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants.
1998,
Pubmed
Perrino,
Calcium regulation of calcineurin phosphatase activity by its B subunit and calmodulin. Role of the autoinhibitory domain.
1995,
Pubmed
Perrino,
Characterization of the phosphatase activity of a baculovirus-expressed calcineurin A isoform.
1992,
Pubmed
Rao,
Transcription factors of the NFAT family: regulation and function.
1997,
Pubmed
Schreiber,
The mechanism of action of cyclosporin A and FK506.
1992,
Pubmed
Tong,
Synaptic desensitization of NMDA receptors by calcineurin.
1995,
Pubmed
Trewavas,
How plants learn.
1999,
Pubmed
Trewavas,
Transduction of Ca2+ signals in plant cells and compartmentalization of the Ca2+ signal.
1996,
Pubmed
Watanabe,
Activation of calcineurin A subunit phosphatase activity by its calcium-binding B subunit.
1996,
Pubmed
Withee,
An essential role of the yeast pheromone-induced Ca2+ signal is to activate calcineurin.
1997,
Pubmed
Yamaguchi-Shinozaki,
A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
1994,
Pubmed
